Papers by Keyword: Microcarriers

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Authors: J. Feng, M. Chong, J. Chan, Z.Y. Zhang, S.H. Teoh, Eng San Thian
Abstract: The current available microcarriers were mainly targeted towards pharmaceutical industries, and might not be suitable for therapeutic implantation. As such, apatite-based microcarriers intended for bone tissue engineering applications would be featured here. Hydroxyapatite-Alginate (HA-Alg) suspension was extruded drop-wise into a calcium chloride (CaCl2) crosslinking solution. The HA-Alg microcarriers were then sintered to form microcarriers of uniform size. The physicochemical properties were analysed by scanning electron microscopy (SEM), X-ray diffractometery (XRD), and fourier transform infrared (FTIR) spectrophotometry. Cell viability on these microcarriers was evaluated using human fetal mesenchymal stem cells (hfMSCs). SEM images revealed that sintered apatite-based microcarriers exhibited a rough surface topology with interconnected pores. XRD results showed that these microcarriers remained phase pure since no other secondary calcium phosphate phases were detected. FTIR analysis indicated several sharp phosphate bands coupled with a hydroxyl band (all belonging to HA). Live/dead staining showed that hfMSCs remained viable after 14 days of culture, and cells have spread and covered the surfaces of the microcarriers. Certainly, these cell-loaded microcarriers could be potentially used in bone implant science.
Authors: Ju Hee Ryu, Byoung Soo Kim
Abstract: Previously, we have developed a novel method for suspension culture of anchoragedependent animal cells using biodegradable polymer nanospheres. In this study, we compared the polymer nanosphere culture method to dextran microcarrier culture method, which is a conventional suspension culture method. Most of human dermal fibroblasts (91 ± 5 %) cultured with polymer nanospheres formed aggregates on day 2. Most of cells (92 ± 7 %) attached onto microcarriers by 4 h. Microcarrier culture method had a lower apoptotic activity (3.4 folds on day 4), compared to the nanosphere culture. The microcarrier culture method had a higher cell growth (2.4-fold versus 1.7- fold growth on day 4) than the nanosphere culture. Although the polymer nanosphere culture method did not yield better outcomes than the microcarrier culture, the polymer nanosphere culture method may offer advantages over the microcarrier culture method with respect to cell protection from the shear stress during agitation at high speed and cell transplantation without enzyme digestion process to harvest cultured cells.
Authors: Lei Ye, Shu Ding, Yuan Lu Cui, Qiang Song Wang, Ye Zhang
Abstract: This article presents the optimization of process parameters in chitosan-gelatin composite microcarriers preparation based on multi-index test breakdown formula evaluation combined with orthogonal array. In this study, the concentration of crosslinker solution, the concentration of water phase and stirring speed were considered as controllable factors, and three levels for each of these factors were selected, in an L9 orthogonal array. The optimal levels of the process parameters were determined through the range analysis and the relative importance among the process parameters were identified through analysis of variance. According to the evaluations of particle size, morphological analysis, compressibility, and equilibrium swelling from the nine different sets, the optimum combinations for microcarriers preparation were showed as: the concentration of crosslinker solution:0.5% (wt/v), the concentration of water phase:5% (wt/v) and stirring speed:240 rpm.
Authors: Gabriela A. Silva, Olga P. Coutinho, Rui L. Reis
Abstract: In the present work we describe the synthesis of starch-based/BG 45S5 particles and their in vitro bioactivity behaviour by means of immersion in a simulated body fluid. The composite particles have shown to form a layer of Ca-P at their surface, whose nature was confirmed by chemical and morphological analysis. In order to evaluate the ability of these particles to be used as carriers for cell culture, undifferentiated rat cells were selected and parameters like cell adhesion, proliferation and expression of osteoblastic markers, were evaluated. The starch-based micro-particles have shown to support cellular activity, allowing cells to attach, proliferate and express specific markers while cultured in the particles surface. The final goal is to be able to use these particles as carriers for cells and simultaneously as in-situ forming constructs for tissue engineering and regenerative medicine applications.
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