Papers by Keyword: Microscope

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Authors: Xue Li Zhang, Cun Wei Lu
Abstract: The aim of our research is to realize a high-sensitivity three-dimensional (3-D) shape measurement of target with an optical microscope. On the conventional method – shape from focus, it’s to regulate focal length of microscope to obtain 3-D shape information of the specimen. However, the method is used the vertical stratification method to obtain 3-D shape information of total surface of target, and the variable focal length of zoom lens is limited therefore the measurement accurate is confined on the vertical direction. In order to solve these problems, we propose a high-sensitivity 3-D shape measurement method based on microscope and laser projection. The method is based on the slit pattern projection technique and 3-D image processing technique. The proposal method can be used for medicine, pharmacy, life science, and material science.
Authors: Irina G. Palchikova, Evgenii S. Smirnov, Alexander A. Konev
Abstract: Comparison of quantitative methods for the segmentation of nuclei images is performed. A sizeable variability is typical for biological specimens and it induces the elemental uncertainty in experimental data. To remove the variability from comparison we have proposed and built the special test objects of two types simulating the nuclei images. I-type objects are test patterns as sets of circles and squares of specified dimensions in chromium films on a glass plate. II-type objects are images with brightness differentials that simulate diffractive blurring and are built up with MathCad programming environment. Test objects enable objective comparison of characteristics obtained in the course of their quantitative optical-and-structural analysis using various algorithms and programs. We found that the efficiency of the segmentation algorithm depends on diffractive blurring of the image. Specifics of Otsu’s algorithm and local algorithm of brightness gradient are analyzed for finding the segmentation threshold of digital images modeling transmission Feulgen-stained cell nuclei specimens with diffractive blurring. The performed calculations revealed that the border of geometrooptical image practically coincide with the points of inflection on the intensity distribution graph in a test-object image space. Computational experiments show that quantitative results of the morphometric image study defined by the various segmentation algorithms vary within 5%. It is established that the threshold-identifying algorithm based on the brightness gradient is preferable in the image cytometry.
Authors: Sha Long, Yan Tong, Fu Xuan Shen, Hong Ke Ai, An Hou Long
Abstract: The mechanism for phosphate cement hydraulic reaction is determined. The microscopic process is described by means of scanning electronic microscope, X-ray diffraction analysis,infrared spectrophotometry and differential heat analysis. The results show the three main phases are present, i.e. hydromagnesium phosphates, Mg (OH)2 and MgO phase. The type, structure, and amount of every phase play an important role in phosphate cement properties. Hydromagnesium phosphate phase structure has a close relation to phosphate cement setting and hardening. The development of strength has much to do with the formed crystalline directing.
Authors: Shigeyuki Haruyama, Didik Nurhadiyanto, Ken Kaminishi
Abstract: Contact width is important design parameter for optimizing design of new metal gasket. The contact width was found have relationship with helium leak quantity. Increasing axial force, the contact width will increase and helium leakage will decrease. This study we conducted the surface roughness evaluation of 25A-size metal gasket before and after use. The results denote the real contact width after contact with flange having different surface roughness. The real contact width for the flange having smoother surface roughness is wider than the rougher one.
Authors: Kai Xiang Li, Ji Hong Feng, Hao He, Hua Juan Bai
Abstract: The purpose of this paper is to put up a device for urinary sediment detection with a homemade microscope and a CCD, and to test the feasibility of the device. In this study, we use a capillary to absorb the urinalysis control (UC) which is diluted 500 times, and drip it into a counting pool of a urinary sediment quantitative analysis board (USQAB). In this setup device, we detect 30 counting pools in this analysis board with microscopic examination in total. Further, we count the number of red blood cells (RBC) and white blood cells (WBC) of every counting pool, and calculate the average number of RBC and WBC of 30 counting pools. Though this detection device, we are able to get 30 groups of imaging results clearly while the number of RBC and WBC in each counting pool fluctuates around the mean value. The result indicates that the setup detection device in this experiment is simple and feasible.
Authors: Pavel Solfronk, Jiří Sobotka, Michaela Kolnerová, Lukáš Zuzánek
Abstract: These days are in the automotive industry going to be still more and more used new types of protective coatings on the Zn-Mg basis. The advantage of these coatings is their excellent protection against corrosion of basic material. On the other hand disadvantage is mainly their low formability which takes effect at own stamping process. Such paper deals with the research influence of the deformation on the damage process of this coating. Size of deformation at different stress states was measured with the help of photogrammetry (system ARAMIS). Coating damage process at these stress states was monitored by means of optical and electron microscopy. Results of protective coating Zn-Mg damage process are presented in the form of strain distribution maps and images from optical and electron microscope.
Authors: Chao Ming Lin, Yung Chuan Chiou, Chun Yi Chu
Abstract: Anisotropic conductive film (ACF), is a lead-free material that is commonly used in fine-pitch interconnect manufacturing to make and maintain the electrical and mechanical connections between the micro-electrodes. A key issue about the circuit conductivity is the deformation, breakage, and number of conductive particles in the ACF packaging. For the field of vision, the Film-On-Glass (FOG) assembly on the glass-side is used to obtain excellent images in experimental observation. This paper utilizes the microscope technology to investigate the bonding properties of the conductive particles, and consider the electrical resistance effects after packaging. The results show the deformation shape, breakage type, and number of conductive particles will be quantitatively affect the electrical performances, and one can measure the area, diameter, and roundness of the deformed particles’ projection in the glass-side view to evaluate the ACF packaging quality.
Authors: Zhe Chuan Feng, S.J. Chua, A.G. Evans, John W. Steeds, K.P.J. Williams, G.D. Pitt
Authors: Zhe Chuan Feng, M. Schurman, C.A. Tran, T. Salagaj, B. Karlicek, Ian T. Ferguson, R.A. Stall, C.D. Dyer, K.P.J. Williams, G.D. Pitt
Authors: Qing Meng Wang, Hua Feng Qin, Qing Song Liu, Tao Song
Abstract: A microscope to image weak magnetic fields using a low-temperature superconducting quantum interference device (SQUID) had developed with a liquid helium consumption rate of ~0.5L/hour. The gradient pickup coil is made by a low-temperature superconducting niobium wire with a diameter of 66 μm, which is coupled to the input circuit of the SQUID and is then enwound on the sapphire bobbin. Both of the pickup coil and the SQUID sensor are installed in a red copper cold finger, which is thermally anchored to the liquid helium evaporation platform in the vacuum space of the cryostat. To reduce the distance between the pickup coil and sample, a 100 μm thick sapphire window is nestled up to the bottom of the cryostat. A three-dimensional scanning stage platform with a 50 cm Teflon sample rack under the sapphire window had the precision of 10 μm. To test the fidelity of the new facility, the distribution of the magnetic field of basalt slice specimens was determined. Results show that the spatial resolution of the newly-designed facility is 500 μm with a gradient magnetic field sensitivity of 380 fT. This opens new opportunities in examining the distribution of magnetic assemblages in samples, which bear great geological and geophysical information.
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