Papers by Keyword: Proliferation

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Authors: Gigliola Lusvardi, Ginaluca Malavasi, Ledi Menabue, Maria Cristina Menziani
Abstract: This review presents a combined experimental-computational strategy for the development of potential bioactive zinc–containing silicate glasses and shows how sound relationships among the structural role of some key elements that appear to control bioactivity can by established and exploit for rational glass design.
Authors: Frank Lüthen, Claudia Bergemann, Ulrike Bulnheim, Cornelia Prinz, Hans Georg Neumann, Andreas Podbielski, Rainer Bader, Joachim Rychly
Abstract: To stimulate bone regeneration, the design of bioactive implants is a great challenge in current orthopedic research. We reasoned that implants should be suitable both to stimulate osteogenic differentiation of mesenchymal stem cells and prevent infections at the site of implantation. Therefore, we focus on copper ions, which are known to exert antimicrobial effects. On the other hand, copper is essential for the cell physiology, including the formation of the extracellular matrix. We studied the influence of copper ions on mesenchymal stem cells at various concentrations and identified the limits of copper concentrations for cell survival. Below the critical concentration for cell survival we analysed proliferation and osteogenic differentiation of the cells in the presence of copper ions. We found that copper stimulated the proliferation of the mesemchymal stem cells at 0.1 mM. Osteogenic differentiation decreased after 14 days at a concentration of 0.05 - 0.1 mM copper ions in osteogenic medium measured by the expression of osteogenic proteins, like alkaline phosphatase (ALP), bone sialoprotein (BSP) and collagen I (COL). We argue that at the implant surface a higher concentration of copper could prevent biofilm formation of bacteria and physiological concentrations in the vicinity of the implant would stimulate stem cell expansion. Together, copper is an interesting agent to control both bacteria and stem cells in the field of implant technology.
Authors: Priya Saravanapavan, S. Verrier, Larry L. Hench
Authors: P.P. Chen, Sheng Min Zhang, L. Cheng, S.L. Huang, Jian Liu, W. Zhou, H. Gong, Q.M. Luo
Abstract: In this paper, three different scale HA/PDLLA porous scaffolds, nano-HA/PDLLA, micro-HA/PDLLA and pure PDLLA were successfully fabricated using solvent casting/particulate leaching method. Chondrocytes adhesion and proliferation on these scaffolds were investigated. In detail, the cells attachment rate and proliferation on nano-HA/PDLLA, micro-HA/PDLLA and pure PDLLA were quantitatively evaluated by cytometry. The interaction between the scaffolds and chondrocytes were observed by optical microscope with HE staining and FE-SEM. The results exhibited that nano-HA/PDLLA scaffold has a modified cell adhesion property, and cells on the nano-scaffold grow much better both in biological and morphological characteristics than on the micro-HA/PDLLA and pure PDLLA scaffolds. This work suggested that nano-HA/PDLLA composite scaffold can significantly improved cell adhesion and proliferation tendency with the existing of nano-effects,and could be used as a potential scaffold material for bone defect repair.
Authors: Takao Saito, Hikoshiro Hayashi, K. Uoe, Takashi Kizuki, Kay Teraoka, Katsuya Kato, Yoshiyuki Yokogawa
Abstract: Our experiments of mouse osteoblast-like MC3T3-E1 cells cultured on a glass substrate showed that as surface roughness of a substrate increased, cell proliferation, cell differentiation and subsequent mineralization were reduced.
Authors: Anton L. Popov, Olga G. Tatarnikova, Nelly R. Popova, Irina I. Selezneva, Azamat Y. Akkizov, Artem M. Ermakov, Olga S. Ivanova, Vladimir K. Ivanov
Abstract: One of the main reasons for limiting the widespread clinical use of mesenchymal stem cells (MSCs) is a low speed of their proliferation in vitro. In this regard, the search for new safe and effective growth stimulants is an urgent task. In this study, we investigated the effect of nanocrystalline cerium oxide doped with gadolinium (Ce1-х Gdх Oy), on the morphofunctional characteristics and proliferative activity of MSCs derived from dental pulp. It was shown that the introduction of Ce1-х Gdх Oy nanoparticles into the culture of dental MSCs provides the activation of proliferation of the cells in a dose-dependent manner. High concentrations of Ce1-х Gdх Oy nanoparticles inhibit the proliferation of the cells; however, this does not lead to further development of apoptosis and cell death. The obtained results indicate that the nanocrystalline cerium oxide can be considered as a basis for the development of highly efficient and low-cost supplements for culturing MSCs.
Authors: Dae Joon Kim, Jung Suk Han, Young Jun Lim, Kyung Soo Jang, Chang-Young Ahn
Abstract: The biocompatibility of zirconia-alumina composite was evaluated with HOS osteoblast like cell models. A total of 18 zirconia-alumina composite disc (diameter: 19mm and thickness: 1.5mm) were prepared and divided into two groups. Half of the discs were sandblasted with 50µm alumina particles. Mean values of surface roughness (Ra) were 0.1 µm and 0.9-1.48 µm for smooth and sandblasted sample respectively. The cell attachment, proliferation, and differentiation on the specimen were evaluated by the Real-Time Polymerase Chain Reaction (RT-PCR), Methylthiazole Sulfate (MTS) analysis, Alkaline Phosphatase (ALP) activity, and Scanning Electron Microscopy (SEM). There was no significant difference in cellular response between two groups. The analysis of the RT-PCR showed that the amount of Cyclin D1(mRNA expression) was not statistically significant different between two groups after 24 hours as well, however markedly decreased in the smooth surface after 72 hours. This indicated that the rough one might have a more favorable cellular proliferation compared to smooth one in long term evaluation. Further study will be necessary.
Authors: Takao Saito, Hikoshiro Hayashi, Tetsuya Kameyama, Katsuya Kato
Abstract: MC3T3-E1 mouse osteoblast-like cells were seeded at high cell density to form confluent monolayer on rough surfaced culture substrates. Osteoblastic gene and protein expressions and matrix mineralization were investigated to clarify the effect of surface roughness on differentiation of MC3T3-E1 cells.
Authors: Jun Yang, Chun Chu, Biao Deng, Sai Liang Ding, Guang Hui Wang, Yong Zhang, Zhi Hua Quan
Abstract: Abstract Objective To testify the special cytotoxicity of TGF-alpha-SAP on proliferating vascular smooth muscle cells and endothelial cells. Methods Conjugation of saporin to TGF-alpha was accomplished after derivatization of saporin and TGF-alpha with N-succinimidyl-3 (2-pyridyldithio) proprionate and final purification of the conjugate was achieved within Eppendorf Centrifugal Filter Cytotoxicity assays were measured by cell count. The studies of influence of TGF-alpha-SAP on values of Thymidine and leucine incorporation into SMCs and ECs were measured by 3H-thymidine uptake and 3H-leucine uptake, respectively. and receptor competition studies of TGF-alpha-SAP are measured by adding excess TGF-alpha in SMCs exposed for TGF-SAP. Results Cytotoxicity assays testified TGF-alpha-SAP conjugate could inhibit remarkably proliferation of SMCs in culture. The values of thymidine of TGF-alpha-SAP group (10-9M and 10-7M) in comparison significantly decreased to 60.9% and 56.0% of the control group respectively, suggesting that cellular DNA synthesis obviously decreased as TGF-alpha-SAP was added. But Saporin did not affect cellular DNA synthesis at higher level. The rate of 3H-leucine incorporation of TGF-alpha-SAP group significantly decreased to 47.3% of the control group, suggesting that SMCs protein synthesis obviously decreased as TGF-alpha-SAP was added. But TGF-alpha-SAP at the same level did not affect DNA synthesis and protein synthesis of ECs compared with the control group. Conclusion The results indicated that TGF-alpha-SAP possesses the more effective cytotoxicity than Saporin and the more special citotoxicity on proliferating vascular smooth muscle cells than on proliferating endothelial cells. Key words:cytotoxicity;selective; Drug-eluting stents;proliferation;saporin
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