Papers by Keyword: VEGF

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Authors: Zi Chao Li, Xiao Wen Li, Jian Ping Wang, Jin Yi Zhong
Abstract: To study the effect of Angelica keiskei chalcone (AC) on microvessel density (MVD) and vascular endothelial growth factor (VEGF) expression on mice hepatocarcinoma cells. Fifty mice were inoculated with hepatocarcinoma H22 cells and divided into 5 groups. Group one to three were administered orally with AC by 5, 25 and 50 mg/kg/d, respectively. Group four was given Endostar 4mg/kg/d by intraperitoneal injection and tumor control group (group five) was given with normal saline. All mice were sacrificed after 10 days. The proliferation activity of hepatocarcinoma cells was determined by methy tetrazolium (MTT) assay, and the levels of the MVD and VEGF protein expression were detected by immunohistochemistry method. The AC inhibitory rates for tumor size were 4.20%, 30.47% and 39.42% at AC treatment dose of 5, 25, and 50 mg/kg, respectively. The average MVD count was 14.2, 11.2 and 8.5 at treatment dose of 5 mg/kg, 25 mg/kg and 50 mg/kg AC, respectively. The protein levels of VEGF in mice treated with 25 mg/kg and 50 mg/kg AC were significantly decreased. The results showed that AC could inhibit tumor angiogenesis effectively and the inhibition mechanism might be associated with the down-regulation of VEGF.
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Authors: Chun Guang Duan, Guo Lin Meng, Jian Liu, Yun Yu Hu, Bao Feng Li, Dan Li, Jian Ping Bai, Long Bi, Min Liu
Abstract: Large osseous defects are difficult to treat because of deficient blood supply on the defected area. To get sufficient blood supply, we designed to establish the adenovirus simultaneously encoding both VEGF and Ang-1 (pAd-VIA) to accelerate the formation of new vessels in the process of bone defect repair. The construction of the adenovirus was performed according to the method reported by Tong-Chuan HE with a tiny modification. Three kinds of adenoviruses were acquired. They are adenovirus pAd-VIA simultaneously encoding VEGF and Ang-1, adenovirus pAd-VEGF encoding VEGF, and adenovirus pAd-Ang-1 encoding Ang-1. The adenovirus prepared in this study could successfully transfer VEGF and Ang-1 into mesenchymal stem cells(MSCs) with high efficiency. Two-gene modified artificial bone was established by use of these adenovirus. In the end, the two-gene modified artificial bone was proved to have good biocompatibility and biological function. Study reports presented here will pave the way for further exploration of vascularization in the process of large osseous defects repair.
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Authors: Hyeong In Kim, Ji Yeon Seo, Seung Jo Jeung, Sae Gwang Park, Young Il Yang
Abstract: Fibrin is a natural substrate for growth, adhesion, and migration of mature endothelial cells (ECs) and a candidate coating material in approaches to graft endothelialization. Adipose tissue represents an abundant, practical source of donor tissue for stem cells which may be a useful source for engineering of vascular grafts. However, the optimal substrates that promote differentiation of adipose tissue-derived stem cells (ASCs) into ECs remain to be elucidated. In the present study, we investigated whether fibrin can be used as a substratum to support in vitro ECs differentiation of ASCs and whether fibrinogen concentration can be affect on ECs differentiation of ASCs. For determination of phenotypic characteristics of ASCs used in this experiment, we performed flow cytometry analysis. ASCs were plated on fibrin composed of varying concentrations of fibrinogen and induced into ECs differentiation in presence of VEGF. Before inducing into ECs, ASCs did not express any markers of hematopoietic cells (CD34, CD45), ECs (CD31, CD34), and endothelial progenitor cells (CD34, CD133, Flk-1). The degree of ECs differentiation was determined by capillary network formation, ECs-specific gene expression, and F-actin assembly. During the first 12 h after seeding, cells spread randomly, moved and formed small interconnected clusters. These clusters decreased in size and formed a capillary tube at 48 h. During the further incubation in presence of VEGF for 7 days, ASCs expressed mRNA and protein of von Willebrand factor (vWF). The degree of ECs differentiation of ASCs was consistently decreased as fibrinogen concentration increase. Fibrin may be used as biomatrix to promote differentiation of ASCs into ECs for tissue engineering.
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Authors: Chan Wai Chan, H.Y. Yeung, K.M. Lee, Y.M. Chiu, X. Guo, P. Chow, Yasuhiko Tabata, Jack C.Y. Cheng
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Authors: Wang Song, Jin Xing
Abstract: Objective To investigate the expression of HIF-la in entochondrostosis,and to study its role in the process of vascular intrusion.Methods The morphology change in rats femoral bone development were observed using HE staining method.The protein expression Of HIF-Ia and VEGF in femoral bone was detected by immunohistochemistry,and the mRNA expression of HIF-Ia and VEGF was detected by reverse transcription-polymerase chain reaction (RT-PCR) method.Results: Many cartilage cells were seen in femoral bone at the 1st day after the birth;many vessels began to invade at the 7th dayfat the 13th day,the cartilage matrix began to calcification,vessels and cartilage cells were reduced.The protein expression of HIF-la and VEGF was increased at the 7th daywhile decreased at the 13th day.The mRNA expression of HIF-la and VEGF went up to the maximum at 7th day the time,and then decreased.Conclusion During femoral bone development,hypoxia may induce HIF-I expression,then activates its downstream gene VEGF expression,which would induce the intrusion of exogenous vascular.Promote endochondral osteogenesis.
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Authors: Larry L. Hench, Aldo Roberto Boccaccini, Richard M. Day, Simon M. Gabe
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