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Organization of Photosynthetic Antenna Complex in Lipid Bilayers

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Abstract:

Bacterial photosynthetic antenna complexes are composed of α-helical hydrophobic polypeptides and pigments (e.g., bacteriochlorophyll a). We report here self-assembling properties of an engineered hydrophobic polypeptide with zinc-substituted bacteriochlorophyll a ([Zn]-BChl a) in various lipid bilayer to investigate the effect of lipid species on the self-assembling properties. When the polypeptide and [Zn]-BChl a were mixed in surfactant solution (n-octyl β-D-glucopyranoside: OG) at 25°C, the absorption band [Zn]-BChl a was red-shifted from 770 to 812 nm, that is assignable to quasi-dimeric “subunit-type” complex. By subsequent dilution and cooling of the solution, the absorption band further red-shifted to 836 nm indicative for progressed assembly, ‘LH1-type’ complex. When the subunit-type complex was assembled in lipid bilayer, e.g., phosphatidylcholines (PC’s) and phosphatidylglycerol (PG’s), the assembling property to form LH1-type complex prominently depended on the character of their fatty acid chains in the phospholipids. The subunit complex remained in the fluidic bilayers, but further assembled to form LH1-type complex in solid-like phospholipid bilayers, suggesting that intermolecular force of phospholipid governs the assembling of the polypeptide/[Zn]-BChl a complex.

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AICAM 2005 View Preview

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Periodical:

Advanced Materials Research (Volumes 11-12)

Pages:

623-626

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.11-12.623

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Online since:

February 2006

Authors:

Takehisa Dewa, K. Yoshida, M. Sugimoto, R. Sugiura, M. Nango

Keywords:

Hydrophobic Polypeptide, Lipid Bilayer, Photosynthesis, Self-Assembly

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© 2006 Trans Tech Publications Ltd. All Rights Reserved

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References

[1] R.B. Gennis, in: Biomembranes: Molecular Structure and Function, Springer-Verlag, NY (1989), Chapter 5.

Google Scholar

[2] P.L. Yeagle, in: The Structrure of Biological Membranes (2nd Ed. ), CRC Press, NY (2005), Chapter 15.

Google Scholar

[3] B. Ke, in: Photosynthesis, Photobiochemistry and photobiophysics Advances in Photosynthesis Vol. 10, Kluwer Academic Publishers, Dordrecht (2001), Chapter 1-8.

DOI: 10.1007/s11120-004-3141-9

Google Scholar

[4] A.W. Roszak, T.D. Howard, J. Southall, A.T. Gardiner, C.J. Law, N.W. Isaacs and R.J. Cogdell: Science Vol. 302 (2003), p. (1969).

Google Scholar

[5] S. Bahatyrova, R.N. Frese, C.A. Siebert, J.D. Olsen, K.O. van der Werf, R. van Grondelle, R.A. Niederman, P.A. Bullough, C. Otto and C.N. Hunter: Nature Vol. 430 (2004), p.1058.

DOI: 10.1038/nature02823

Google Scholar

[6] T. Dewa, T. Yamada, M. Ogawa, M. Sugimoto, T. Mizuno, K. Yoshida, Y. Nakao, M. Kondo, K. Iida, K. Yamashita, T. Tanaka and M. Nango: Biochem. Vol. 44 (2005), p.5129.

Google Scholar

[7] K.A. Meadows, K. Iida, K. Tsuda, P.A. Recchia, B.A. Heler, B. Antonio, M. Nango and P.A. Loach: Biochem. Vol. 34 (1995), p.1559.

Google Scholar

[8] C.M. Davis, P.S. Parkes-Loach, C.K. Cook, K.A. Meadows, M. Bandilla, H. Scheer and P.A. Loach: Biochem. Vol. 35 (1996), p.3072.

DOI: 10.1021/bi951777l

Google Scholar

[9] G. Cevc, in: Phospholipids Handbook, Marcel Dekker, Inc, NY (1993), p.498.

Google Scholar