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The Effect of 3D Construction Culture of Human Chondrocytes Using Alginate Sponge

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Abstract:

In this study, we investigated the effect of the use of alginate sponge as a chondrocyte-3D scaffold for the construction of a cartilage graft. Alginate sponge was made by 5% alginic acid which was crosslinked by CaCl2. Chondrocytes were obtained from a nasal septum after the operation and cultured in 3D alginate sponge. For analysis of cell differentiation, we have checked aggrecan, collagen type I and II using RT-PCR and performed the histological and scanning electron microscopy analysis. Our experiments showed that alginate sponge of 5% promoted sufficient chondrocyte proliferation and differentiation, resulting in the formation of a specific cartilage matrix. The sponge presents new perspectives with respect to in vitro production of "artificial" cartilage. We conclude that the alginate sponges have potential as a scaffold for cartilage tissue engineering.

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Periodical:

Key Engineering Materials (Volumes 326-328)

Pages:

883-888

DOI:

https://doi.org/10.4028/www.scientific.net/KEM.326-328.883

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Online since:

December 2006

Authors:

Jin Sang Lee, Byung Kim, Min Soo Kim, Seung Jae Lee, Sung Won Kim, Dong Woo Cho, Joo Sung Kim, Geun Bae Lim

Keywords:

Alginate Sponge, Chondrocyte, Scaffold, Tissue Engineering

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© 2006 Trans Tech Publications Ltd. All Rights Reserved

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