Controlled Crystallization of Tooth-Like Hydroxyapatite under Gelatin Monolayer

Kun Tian; Dong Hua Guan; Ping Wu; Chun Peng Huang; Lin Niu; Su Qin Xian; Yu Chen; Peng Wang; Yi Li Qu; Yong Mei Ye; Ting Ting Wang; Zhi Qing Chen

doi:10.4028/www.scientific.net/KEM.330-332.663

Paper Titles

Ultra-Thin Film of Chitosan and Sulfated Chitosan Coating on Titanium Oxide by Layer-by-Layer Self-Assembly Method
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Characterization of Hydroxyapatite Sputtered Films Doped with Titanium
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Exploring Antibacterial and Antiadhesive Activities of Titanium Surface Modified with Hydroxyapatite Sol-Gel Containing Silver
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Formation of a Laminin–Apatite Composite Layer with Low Surface Roughness on a Polymer Surface
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Controlled Crystallization of Tooth-Like Hydroxyapatite under Gelatin Monolayer
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The Effect of Titania Deposited by Hydrolysis at Low Temperature on Aptite Formation Ability in DMEM
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Bone-Like Apatite Formation on Modified PCL Surfaces under Different Conditions
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Apatite-Forming Ability of Pectin Gels in Simulated Body Environment
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Bonelike Apatite Formation on Synthetic Organic Polymers and Fiber Coated with Titania
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HomeKey Engineering MaterialsKey Engineering Materials Vols. 330-332Controlled Crystallization of Tooth-Like...

Controlled Crystallization of Tooth-Like Hydroxyapatite under Gelatin Monolayer

Abstract:

Based on the molecular recognition theory, an organic molecules model was designed to induce the hydroxyapatite crystallization, to build a tooth-like calcium phosphate/hydroxyapatite under a controllable way in vitro. The cross-linking of collagen on the dentin surface and gelatin was optimized by varying the molar ratio of N,N-(3-dimethylaminopropyl)- N'-ethyl-carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) at a constant EDC concentration. CaCl2 and Na3PO4-12H2O solutions were added after the crosslinking process. The whole process requires repeating the crosslinking and mineralization process for five times. The obtained composite were characterized by X-ray diffraction (XRD) and scanning electron microscopy (SEM) as well as energy dispersive X-ray photoelectron spectroscopy (XPS). The results showed that the dentinal tubule were blocked by neonatal hydroxyapatite layer which has a continuous structure of columns crystal with size of 10-40nm. Furthermore, there was column crystal with parallel direction inside, similar to the crystal array in the top of enamel rod. This study showed that the specific organic molecule model can be used as a potential effective crystal growth modifier.