Paper Titles
In Vivo and In Vitro Tendon Engineering
p.9
Microscopic Multi-Directional Mechanical Properties of Human Femoral Cancellous Bone Tissue
p.13
Fibrinogen Concentration Influence on VEGF-Induced Differentiation of Adipose Tissue-Derived Stem Cells into Endothelial Cells
p.17
Fibroblasts-Populated Fibrin Membrane Enhance Wound Closure
p.21
Development of Efficient Cardiac Differentiation Method of Mouse Embryonic Stem Cells
p.25
Bone Grafting Using Particulate Dentin
p.29
Porous Polymer Prosthesis for Meniscal Regeneration
p.33
Effects of Hormone Treatment on the Mechanical Characteristics of Osteoporotic Vertebral Trabecular Bone Using RP and FE Models
p.37
Relationship of Morphological and Mechanical Characteristics in Vertebral Trabecular Bone
p.41

Development of Efficient Cardiac Differentiation Method of Mouse Embryonic Stem Cells

Article Preview

Abstract:

To obtain an enhanced population of cardiomyocytes from differentiating mouse embryonic stem (ES) cells, we confirmed the role of noggin treatment during the cardiac differentiation of mouse ES cells. ES cells were cultured in ES medium containing both noggin and LIF for 3 days on the mouse embryonic fibroblast feeder layer, followed by dissociated and suspension culture without LIF to form the embryoid body (EB). The next day, noggin was eliminated and EBs were cultured continuously. Noggin treated ES cells showed a relatively rapid increase of cardiac marker genes, while the vehicle (PBS) treated group showed no significant cardiac marker expression at 4 days after the EB formation. Furthermore, Noggin treated ES cells showed 68.00±9.16% spontaneous beating EBs at 12 days after the EB formation. To develop a more efficient cardiomyocyte differentiation method, we tested several known cardiogenic reagents (ascorbic acid, 5’-Azacytidine, LiCl, oxytocin, FGF2 and PDGF-BB) after noggin induction or we cultured noggin treated ES cells on various extracellular matrixes (collagen, fibronectin and Matrigel). Quantitative RT-PCR and immunocytochemistry results showed a significantly increased cardiac differentiation rate in the FGF2 treated group. Differentiation on the collagen extracellular matrix (ECM) could slightly increase the cardiac differentiation efficiency. These results show the possibilities for the establishment of selective differentiation conditions for the cardiac differentiation of mouse ES cells.

You might also be interested in these eBooks
Advanced Biomaterials VII View Preview

Info:

Periodical:

Key Engineering Materials (Volumes 342-343)

Pages:

25-28

DOI:

https://doi.org/10.4028/www.scientific.net/KEM.342-343.25

Citation:

Cite this paper

Online since:

July 2007

Authors:

S. Hong, J.K. Kang, C.J. Bae, E.S. Ryu, S.H. Lee, J.H. Lee

Keywords:

Cardiomyocyte, Differentiation, Embryoid Body, Embryonic Stem Cells, Noggin

Export:

RIS, BibTeX

Price:

Permissions CCC:

Request Permissions

Permissions PLS:

Request Permissions

Сopyright:

© 2007 Trans Tech Publications Ltd. All Rights Reserved

Share:

Citation:

References

[1] S. Hughes: The Journal of pathology Vol. 197 (2002), p.468.

Google Scholar

[2] T. Takahashi, B. Lord, P.C. Schulze, R.M. Fryer, S.S. Sarang, S.R. Gullans and R.T. Lee: Circulation Vol. 107 (2003), p. (1912).

DOI: 10.1161/01.cir.0000064899.53876.a3

Google Scholar

[3] A. Sachinidis, C. Gissel, D. Nierhoff, R. Hippler-Altenburg, H. Sauer, M. Wartenberg and J. Hescheler: Cell Physiol. Biochem. Vol. 13 (2003), p.423.

DOI: 10.1159/000075130

Google Scholar

[4] J. Paquin, B.A. Danalache, M. Jankowski, S.M. McCann and J. Gutkowska: Proceedings of the National Academy of Sciences of the United States of America Vol. 99 (2002), p.9550.

DOI: 10.1073/pnas.152302499

Google Scholar

[5] N. Rosenblatt-Velin, M.G. Lepore, C. Cartoni, F. Beermann and T. Pedrazzini: J. Clin. Invest. Vol. 115 (2005), p.1724.

DOI: 10.1172/jci23418

Google Scholar

[6] M. Koyanagi, J. Haendeler, C. Badorff, R.P. Brandes, J. Hoffmann, P. Pandur, A.M. Zeiher, M. Kuhl and S. Dimmeler: J. Biol. Chem. Vol. 280 (2005), p.16838.

DOI: 10.1074/jbc.m500323200

Google Scholar

[7] C. Xu, S. Police, N. Rao and M.K. Carpenter: Circulation Res. Vol. 91 (2002), p.501.

Google Scholar

[8] S. Yuasa, Y. Itabashi, U. Koshimizu, T. Tanaka, K. Sugimura, M. Kinoshita, F. Hattori, S. Fukami, T. Shimazaki, S. Ogawa et al: Nat. biotech. Vol. 23 (2005), p.607.

DOI: 10.1038/nbt1093

Google Scholar

[9] H. Baharvand, M. Azarnia, K. Parivar and S.K. Ashtiani: J. Mol. Cell. Cardiol. Vol. 38 (2005), p.495.

Google Scholar

[10] M. Okada-Ban, J.P. Thiery and J. Jouanneau: Int. J. Biochem. Cell Biol. Vol. 32 (2000), p.263.

Google Scholar

[11] M.J. Solloway and R.P. Harvey: Cardiovascular Res. Vol. 58 (2003), p.264.

Google Scholar

[12] R.J. Bick, M.B. Snuggs, B.J. Poindexter, L.M. Buja and W.B. Winkle: Cell Adhes. Commun. Vol. 6 (1998), p.301.

Google Scholar

[13] L.L. Hilenski, X.H. Ma, N. Vinson, L. Terracio and T.K. Borg: Cell Motil. Cytoskeleton Vol. 21 (1992), p.87.

DOI: 10.1002/cm.970210202

Google Scholar

[14] L.L. Hilenski, L. Terracio and T.K. Borg: Cell Tissue Res. Vol. 264 (1991), p.577.

Google Scholar

[15] M. Eghbali, O.O. Blumenfeld, S. Seifter, P.M. Buttrick, L.A. Leinwand, T.F. Robinson, M.A. Zern and M.A. Giambrone: J. Mol. Cell. Cardiol. Vol. 21 (1989).

Google Scholar