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In Vitro Chondrocyte Culture Using Hydrophilized PLLA Scaffold in Bioreactor System

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Abstract:

Nonporous PLLA film and porous PLLA scaffolds were prepared and then grafted with acrylic acid (AA) using in situ direct plasma treatment to obtain PLLA-g-PAA. Chondrocytes isolated from rabbit knee articular cartilages were cultivated in Dulbecco’s modified eagle medium- F12 (DMEM-F12) containing 10% fetal bovine serum (FBS) and 1% antibiotics and passaged twice before cell seeding. Once seeded on either PLLA films or scaffolds, they were placed in a bioreactor system and an intermittent hydrodynamic pressure (IHP) was applied in 3 bars, while turned on for 2 min and off for 28 min during 15-day culture. AA grafting to PLLA surface was confirmed from various surface analyses. From WST-1 assay, chondrocyte proliferation was significantly improved with dynamic IHP for PLLA and PLLA-g-PAA scaffolds as compared to static culture. This study indicates that IHP may have significant influence on chondrocytes behavior in 3D culture environment.

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Periodical:

Key Engineering Materials (Volumes 342-343)

Pages:

253-256

DOI:

https://doi.org/10.4028/www.scientific.net/KEM.342-343.253

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Online since:

July 2007

Authors:

Hee Seok Yang, Kwi Deok Park, Byoung Soo Kim, Jae Jin Kim, Kwang Duk Ahn, Dong Keun Han

Keywords:

Acrylic Acid (AA), Bioreactor, Chondrocyte, PLLA, Tissue Engineering

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© 2007 Trans Tech Publications Ltd. All Rights Reserved

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References

[1] Y. B. Bouchet, M. Colon, A. Polotsky, A. H. Shikani, D. S. Hungerford, C. G. Frondoza: J. Biomed. Mater. Res. Vol 52 (2000), p.716.

DOI: 10.1002/1097-4636(20001215)52:4<716::aid-jbm17>3.0.co;2-t

Google Scholar

[2] H. C. Chen, H. -P. Lee, Y. -C. Ho, M. -L. Sung, Y. -C. Hu: Biomaterials Vol 27 (2006), p.3154.

Google Scholar

[3] L. E. Freed, A. P. Hollander, I. Martin, J. R. Barry, R. Langer, and G. Vuniak-Novakovic: Exp. Cell. Res. Vol. 240 (1998), p.58.

Google Scholar

[4] T. Ikenoue. T. C. D. Trindade, M. S. Lee, E. Y. Lin, D. J. Schurman, S. B. Goodman, R. L. Smith: J. Orth. Res. Vol 21 (2003), p.110.

Google Scholar

[5] U. Hansen, M. Schunke, C. Domm, N. Ioannidis, J. Hassenpflug, T. Gehrke, B. Kurz: J. Biomechanics Vol 34 (2001), p.941 �.

DOI: 10.1016/s0021-9290(01)00050-1

Google Scholar