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Effect of Collagen Coating on Lipid Accumulation and Differentiation on 3T3-L1 Preadipocyte Treated with Conjugated Linoleic Acid

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Abstract:

The purpose of this study is to make use of trans10,cis12 CLA (t-CLA) that has potential for proliferation and differentiation to form adipocyte on the collagen-coated surface. Results provided evidences of good adhesion, growth, viability, and differentiation of adipocyte on collagen-coated surface compared with non-coated surface. Also, the results showed that mouse 3T3-L1 preadipocyte can be successfully and reproducibly cultured on the collagen-coated surface, and the adipocyte precursor cells placed on the collagen-coated surface are able to undergo full maturation into adipocytes in the control cells. Moreover, glycerol-3-phosphate dehydrogenase (GPDH) activity in 3T3-L1 preadipocyte cultured on collagen-coated surface with t-CLA was higher than that on polystyrene (PS) surface due to higher cell adhesion and cell viability. These results suggest that collagen coating may provide a promising approach to develop a new adipocyte replacement strategy using CLA.

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Periodical:

Key Engineering Materials (Volumes 342-343)

Pages:

261-264

DOI:

https://doi.org/10.4028/www.scientific.net/KEM.342-343.261

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Online since:

July 2007

Authors:

Hyun Seuk Moon, Hong Gu Lee, Ho Hyun Song, Yun Jaie Choi, Chong Su Cho

Keywords:

Adipocyte Differentiation, Cis12 Conjugated Linoleic Acid (t-CLA), Collagen, Extracellular Matrix, Lipid Accumulation, Trans10

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© 2007 Trans Tech Publications Ltd. All Rights Reserved

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