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Metabolism Peculiarities of Bacteria of the Genus Sulfobacillus
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Isolation, Characterization and Phylogenetic Analysis of Acidiphilium-Like Bacteria from Acid Mine Drainage
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Bacterial Oxidation of Elemental Sulfur: Changes in Oxidation Kinetics
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Quantification of Microorganisms Involved in Cemented Layer Formation in Sulfidic Mine Waste Tailings (Freiberg, Saxony, Germany)
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Analysis of the Microbial Community in the Leachate Collected from an Experimental Bioleaching Column by Cloning and RFLP
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The Diversity of Benthic Microorganisms in Acidic Mine Lake Sediments
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Quantification of Microorganisms Involved in Cemented Layer Formation in Sulfidic Mine Waste Tailings (Freiberg, Saxony, Germany)

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Abstract:

Cemented layers predominantly consisting of gels/poorly crystalline mineral phases have been formed as a consequence of mineral weathering in sulfidic tailings near Freiberg, Saxony, Germany. These layers function as natural attenuation barrier for toxic compounds and reduce oxidation and erosion processes of tailings surfaces. Quantitative molecular biological and cultivation methods were applied to investigate the role of microorganisms for mineral weathering and cemented layer formation. High resolution depth profiles of numbers of microorganisms showed maximal cell numbers in the oxidation zone where cemented layers had been formed. Highest total cell numbers of >109 cells g-1 dry weight (dw) were detected by SybrGreen direct counting. Using quantitative real-time PCR (Q-PCR) between 107 and 109 Bacteria g-1 dw and up to 108 Archaea g-1 dw were determined. As well high numbers of cultivable and living Bacteria could be detected by MPN (most probable number) for Fe(II)- and S-oxidizers and CARD-FISH (catalyzed reporter deposition - fluorescence in situ hybridization). Overall, the high numbers of microorganisms determined with various quantification techniques argue for a significant role of microorganisms in cemented layer formation due to microbial mineral weathering. It is hypothesized that EPS (extracellular polymeric substances) mediate the formation of secondary mineral phases.

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Periodical:

Advanced Materials Research (Volumes 20-21)

Pages:

481-484

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.20-21.481

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Online since:

July 2007

Authors:

Dagmar Kock, Torsten Graupner, Dieter Rammlmair, Axel Schippers

Keywords:

Acid Mine Drainage, CARD-FISH, Cemented Layers, Mine Tailings, Real Time-PCR, SYBR-Green

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© 2007 Trans Tech Publications Ltd. All Rights Reserved

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References

[1] D.K. Nordstrom and C.N. Alpers in: The Environmental Geochemistry of Mineral Deposits, Reviews in Economic Geology, edited by G.S. Plumlee and M.J. Logsdon volume 6A, Society of Economic Geologists, Inc., Littleton, CO (1999).

Google Scholar

[2] M. Ueshima, D. Fortin and M. Kalin: Geomicrobiol. J. Vol. 21 (2004), p.313.

Google Scholar

[3] D. Kock and A. Schippers: Hydrometallurgy Vol. 83 (2006), p.167.

Google Scholar

[4] O. Bruneel, R. Duran, K. Koffe, C. Casiot, A. Fourcans, F. Elbaz-Poulichet and J-C. Personné: Geomicrobiol. J. Vol. 22 (2005), p.249.

DOI: 10.1080/01490450590947805

Google Scholar

[5] N. Diaby, B. Dold, H.R. Pfeiffer, C. Holliger, D.B. Johnson and K.B. Hallberg,: Environ. Microbiol. Vol. 9 (2007), p.298.

Google Scholar

[6] R.H. Johnson, D.W. Blowes, W.D. Robertson and J.L. Jambor: J. Contam. Hydrol. Vol. 41 (2000), p.49.

Google Scholar

[7] R.G. McGregor and D.W. Blowes: J. Geochem. Expl. Vol. 76 (2002), p.195.

Google Scholar

[8] S.E. Gilbert, D.R. Cooke and P. Hollings: Environ. Geol. Vol. 44 (2003), p.687.

Google Scholar

[9] M.C. Moncur, C.J. Ptacek, D.W. Blowes and J.L. Jambor: Appl. Geochem. Vol. 20 (2005), p.639.

Google Scholar

[10] M.R. Gunsinger, C.J. Ptacek, C.J. Blowes and J.L. Jambor: J. Cont. Hydrol. Vol. 83 (2006), p.149.

Google Scholar

[11] W. Sand, T. Gehrke, P. -G. Jozsa and A. Schippers: Hydrometallurgy Vol. 59 (2001), p.159.

Google Scholar

[12] F.G. Ferris, S. Schultze, T.C. Witten, W.S. Fyfe and T.J. Beveridge: Appl. Environ. Microbiol. Vol. 55 (1989), p.1249.

Google Scholar

[13] G. Southam and T.J. Beveridge: Appl. Environ. Microbiol. Vol. 58 (1992), p. (1904).

Google Scholar

[14] T. Graupner, A. Kassahun, D. Rammlmair, J.A. Meima, D. Kock, M. Furche, A. Fiege, A. Schippers and F. Melcher: submitted to Applied Geochemistry.

DOI: 10.1016/j.apgeochem.2007.07.002

Google Scholar

[15] X. Lin, S.G. Wakeham, I.F. Putnam, Y.M. Astor, M.I. Scranton, A.Y. Chistoserdov and G.T. Taylor: Appl. Environ. Microbiol. Vol. 72 (2006), p.2679.

DOI: 10.1128/aem.72.4.2679-2690.2006

Google Scholar