Paper Titles
Isolation, Characterization and Phylogenetic Analysis of Acidiphilium-Like Bacteria from Acid Mine Drainage
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Bacterial Oxidation of Elemental Sulfur: Changes in Oxidation Kinetics
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Quantification of Microorganisms Involved in Cemented Layer Formation in Sulfidic Mine Waste Tailings (Freiberg, Saxony, Germany)
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Analysis of the Microbial Community in the Leachate Collected from an Experimental Bioleaching Column by Cloning and RFLP
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The Diversity of Benthic Microorganisms in Acidic Mine Lake Sediments
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Microbial Diversity in an Iron Oxidation Tank of an AMD Treatment Plant at an Abandoned Sulphur Mine
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Reduction of Soluble and Solid Ferric Iron by Acidiphilium SJH
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Development of an Extremophilic Iron Oxidizing Consortium and a Fixed Film Bioreactor for Generation of Ferric Lixivient
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Enrichment of Chromium-Reducing Bacteria Using Potential-Controlled Electrochemical Cultivation
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The Diversity of Benthic Microorganisms in Acidic Mine Lake Sediments

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Abstract:

The sediment microbial communities of a disused coal mine lake, Lake Kepwari (pH~4.5-5) were studied to understand how the natural microbial processes in an oligotrophic acidic mine lake system influence the iron and sulphur cycles. Most probable number (MPN) viable counts were used to enumerate the benthic bacteria at different depths. MPN results revealed an abundance of bacteria that were capable of growing in sulphate reducing medium with numbers in the range of 1 × 107 – 1 × 108 cells.g-1 of wet sediment. In contrast, MPN results showed much lower numbers of bacteria that were capable of growing in ferric reducing medium with 1 × 102 – 2 × 103 cells.g-1 of wet sediment detected. Serial decimal dilution cultures were used to isolate pure strains of benthic bacteria. Strains HP1, HP2 and HP3 were isolated from benthic lake sediments at 18 m, 0 m and 10 m water depths respectively. 16S rRNA gene sequence analysis of strain HP1 showed that the strain belonged to the genus Enterobacter, strain HP2 belonged to the Order Rhizobiales and strain HP3 belonged to the sub-order Micrococcineae. Denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA gene fragments was used to profile the diversity of the benthic microbial communities at different depths. DGGE profiling of benthic sediments revealed that sediments contained mostly members of the Proteobacteria, Actinobacteria and Firmicutes phyla.

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Periodical:

Advanced Materials Research (Volumes 20-21)

Pages:

489-492

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.20-21.489

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Online since:

July 2007

Authors:

Huynh A. Pham, Carolyn E. Oldham, Jason J. Plumb

Keywords:

Acidic Mining Lake, Benthic Sediments, DGGE, DNA Extraction, Iron Reducing Bacteria, Sulfate-Reducing Bacteria (SRB)

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© 2007 Trans Tech Publications Ltd. All Rights Reserved

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