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Protein Adsorption on Hydroxyapatite Nano-Crystals with Quartz Crystal Microbalance Technique

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Abstract:

Real time adsorption behaviors of six proteins with different isoelectric points on hydroxyapatite (HAp) nanocrystal surfaces have been investigated by using HAp sensors for quartz crystal microbalance with dissipation technique (QCM-D). The dissipation (D)–frequency (f) plots clearly showed that the different types of protein adsorption behaviors; the D-f plots of acidic proteins lie on one straight line with a constant slope under all initial protein concentrations, while those of neutral and basic proteins lie on two straight lines with different slopes. The acidic proteins formed a monolayer, while the neutral and basic proteins could cause conformational changes with the adsorbed amount of proteins. The QCM-D technique with novel HAp nanocrystal sensor is useful for the liquid phase changes of proteins on the surface.

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Info:

Periodical:

Key Engineering Materials (Volumes 361-363)

Pages:

1119-1122

DOI:

https://doi.org/10.4028/www.scientific.net/KEM.361-363.1119

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Online since:

November 2007

Authors:

Tomohiko Yoshioka, Toshiyuki Ikoma, Akira Monkawa, Toru Tonegawa, Dinko Chakarov, B. Kasemo, Nobutaka Hanagata, M. Tanaka

Keywords:

Hydroxyapatite (HAP), Nanocrystal, Protein Adsorption, QCM-D

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© 2008 Trans Tech Publications Ltd. All Rights Reserved

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