Attachment and Proliferation of Human Dermal Fibroblasts onto ECM-Immobilized PLGA Films

Hyun Joo Son; Dong Wook Han; H.H. Kim; Hee Joong Kim; In Seop Lee; Jeong Koo Kim; Jong Chul Park

doi:10.4028/www.scientific.net/KEM.288-289.291

Paper Titles

Influence of Dynamic Flow Speed on Bonelike Apatite Formation in Porous Calcium Phosphate Ceramic in RSBF
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Nucleation and Growth of Bone-Like Apatite on Surfaces of Metals, Ceramics and Polymers in Simulated Body Fluids
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Experimental Study on the Osteoinduction of Calcium Phosphate Biomaterials In Vivo and the Capability of Supporting Osteoblast Proliferation In Vitro
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Influence of Characteristics of Titanium Oxide Film on the Biological Behavior of Cultured Human Umbilical Vein Endothelial Cells (Huvec)
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Attachment and Proliferation of Human Dermal Fibroblasts onto ECM-Immobilized PLGA Films
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Surface Modification for Controlling the Blood-Materials Interface
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Structure and Hemocompatibility of Titanium Oxide Films Synthesized by Continuous or Pulsed DC Magnetron Sputtering
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Immobilization of Albumin and Heparin on the Surface of Ti-O Films for Improving Blood Compatibility
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Iridium Oxide as a Stimulating Neural Electrode Formed by Reactive Magnetron Sputtering
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HomeKey Engineering MaterialsKey Engineering Materials Vols. 288-289Attachment and Proliferation of Human Dermal...

Attachment and Proliferation of Human Dermal Fibroblasts onto ECM-Immobilized PLGA Films

Abstract:

In this study, human dermal fibroblast behaviors onto non-porous PLGA (75:25) films immobilized with 1, 10 and 100 µg/ml collagen (CN) or fibronectin (FN) were investigated according to different cell-seeding densities (1,000, 10,000 and 100,000 cells/ml). Cell attachment and proliferation were assessed using water soluble tetrazolium salt. The results indicated that 1 µg/ml of FN-immobilized PLGA film demonstrated significantly (p < 0.05) superior cellular attachment to the intact PLGA film after 4 hr of incubation. Moreover, the number of attached cells was shown to be directly proportional to that of initially seeded cells. After 48 hr, the cells showed significantly (p < 0.05) higher proliferation onto 1 or 10 µg/ml of FN-immobilized PLGA films than onto other PLGA films, regardless of the initial cell-seeding density. In terms of CN-immobilization, cell proliferation was appreciably increased but it was relatively lower than FN-immobilization. These results suggested that ECM-immobilization can enhance the cell affinity of hydrophobic scaffolds and be used to potential applications for tissue engineering by supporting cell growth.