Head-On Immobilization of DNA Fragments on CVD-Diamond Layers

S. Wenmackers; P. Christiaens; W. Deferme; M. Daenen; K. Haenen; M. Nesládek; Patrick Wagner; V. Vermeeren; L. Michiels; Martin Van de Ven; M. Ameloot; J. Wouters; L. Naelaerts; Z. Mekhalif

doi:10.4028/www.scientific.net/MSF.492-493.267

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Full Form of the Near Tip Field for the Interface Crack between a Piezoelectric Material and a Thin Electrode
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Head-On Immobilization of DNA Fragments on CVD-Diamond Layers
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HomeMaterials Science ForumMaterials Science Forum Vols. 492-493Head-On Immobilization of DNA Fragments on...

Head-On Immobilization of DNA Fragments on CVD-Diamond Layers

Abstract:

Synthetic diamond is regarded as a promising material for biosensors: it forms a stable platform for genetic assays and its biocompatibility opens the possibility for in vivo sensing. In this study the use of a thymidine linker for covalent DNA attachment was evaluated. Contact angle measurements provided a qualitative test of the initially oxidized surface. X-ray photoemission spectroscopy was used for further analysis of the oxides and for monitoring the effect of subsequent chemical treatments. The presence of FITC-labelled DNA was confirmed by confocal fluorescence microscopy. Enzyme linked immunosorbent assays indicated that this DNA was merely adsorbed on the diamond surface instead of covalently bound.

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Materials Science Forum (Volumes 492-493)

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267-272

DOI:

https://doi.org/10.4028/www.scientific.net/MSF.492-493.267

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Online since:

August 2005

Authors:

S. Wenmackers, P. Christiaens, W. Deferme, M. Daenen, K. Haenen, M. Nesládek, Patrick Wagner, V. Vermeeren, L. Michiels, Martin Van de Ven, M. Ameloot, J. Wouters, L. Naelaerts, Z. Mekhalif

Keywords:

Affinity Biosensors, DNA Hybridisation, Synthetic Diamond Layers, X-Ray Photoemission Spectroscopy

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