A Novel Methodology that Combined Fast Chromatography and Antioxidant Activities Screening in Baimai Prescription Research

Abstract:

To tackle the difficult problem of large ethnomedicine compound prescription research in a fast and efficient way, high-speed separation of Baimai prescriptions was performed by automated flash chromatography on 100-g Si flash columns at a flow-rate of 40 mL/min. Petroleum ether, methylene dichloride, ethyl acetate, methanol and water were used in gradient elution solvent system. 69 fractions were obtained as an ethnodrug compounds library. Separation degree of partial fractions were determined by HPLC-UV absorption spectrometry. Antioxidant activities screening of the fractions in 96 well plates was carryied out. Distribution range of fractions with strong scavenging effect of free radical DPPH was identified. Fraction 5-18 and 20 extracted from ethyl acetate exert the strongest antioxidant activities in vitro, and are identified as effective-composite of the prescription in radical scavenging. The results reported here suggest that the methods used may lead to improvements in the research and development of large ethnomedicine compound prescription.

• Periodical: Advanced Materials Research (Volume 699)
• Pages: 349-353
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.699.349
• Online since: May 2013
• Authors: Zi Qian Zhang, Xiao Yu Chen, Yun Xia Duan, Fang Liang, Qing Shan Liu, Duo Ming Zhao
• Keywords: Antioxidant Activity, Compound Prescription, Ethnomedicine, Flash Chromatography

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