Papers by Author: D.H. Yoon

Abstract: To develop a suitable scaffold for tissue-engineered bone regeneration, we compared the efficiency of tissue-engineered bone regeneration according to the porous structure of calcium metaphosphate (CMP) ceramic scaffolds. Each scaffold was prepared with a sponge method and a foam-gel method, respectively. Both scaffolds, having either interconnected trabecular pores formed by the sponge method or fully interconnected globular pores formed by the foam-based technology, were not cytotoxic and elicited neither an immune nor an inflammatory response regardless of geometry and fabrication method. The fully interconnected globular porous scaffold showed more favorable compression strength and facilitated osteogenic repair by favoring cellular attachment and osteogenic differentiation with good osteoconductivity compared to the interconnected trabecular pore structured scaffold. These results suggest that the fully interconnected globular porous structure would be more suitable for both a bone substitute and scaffold for bioactive material-based or cell-based tissue bone regeneration.

Abstract: For effective bone regeneration, various surface modifications have been tried. In an effort to improve osteogenic repair potential, we evaluated recombinant peptides containing the RGD domain as a bioactive molecule for tissue-engineered bone regeneration. The synthetic peptides slightly suppressed cellular proliferation in the in vitro culture system but induced favorable osteoblastic differentiation, which was determined by MTT and ALP activity staining, respectively. The synthetic peptide coated CMP granules, which were implanted into the mandibular bone defects showed more favorable bone repair compared to the non-coated CMP implantation. In addition, there were not any sign of inflammatory reaction. These findings suggest that synthesized peptides containing the RGD domain enhance cellular attachment and osteogenic activity in vivo condition and that the peptide-coated CMP granules can serve as a biocompatible bone substitute.