Papers by Author: Jian Huang

Abstract: The aerobic and anaerobic cyclic activated sludge reactor of A/O process was separated into an A/O domestication segments and organic matter removal ability strengthen segments. In the A/O disposal system, organic carbon degradation, nitrogen and phosphorus removal from municipal waste water in reactor were investigated. Results showed that good performance of the A/O disposal system was achieved and the highest removal efficiencies of COD, TN and TP were 96%, 50% and 75%, respectively. When organic loadings varied from low to high, the removal ability increased and then the hybrid microbe in activated sludge degradation ability became strong. So the cyclic activated sludge was a useful method for eliminate organic matter removal from municipal waste water.

Abstract: In order to investigate the capability of degradation lindane and lignocellulose of a microbial community, the gas chromatography(GC) was used to determine the concentration of lindane. After 18 days cultured, lindane reduced 6.25% more than the control. And the result of gas chromatography and mass spectrometry (GCMS) showed that the species and quantum of fermentation productions changed much in different phase. In order to determine the bacterial composition of the community, clone library was used to clarified bacterial composition. Constructed 16S rDNA clone library showed 60% closest relative among them were known the detailed information. Strain of closest relative of Clostridium may be main contribute to lignocellulose degradation, and closest relative of Proteobacterium may be the main contribute to degradation organochlorine pesticide (lindane).

Abstract: In order to investigate the rice straw lost weight, degradation products and changes of microbial communities in agriculture solid waste degradation course. The normal temperature microbial community could decompose 40% of rice straw gross weight within six days. Propionic acid, ethanol, isopropyl alcohol, 4-Amino-1-butanol, butanoic acid, diethoxydimethyl-silane, lactic acid, ethanol-2,2'-oxybis-, diethyl phthalate and glycerin ten kinds compounds detected by GC-MS in the fermented broth. Degradation products changed lots in different fermented period. For analyzing the diversity of the microbial communities, Denaturing Gradient Gel Electrophoresis (DGGE) were used to identify the composition diversity. Phylogenetic tree derived from 16S rDNA sequence indicated the microbial community have abundant diversity.