Papers by Author: M. Dorget

Abstract: Porous Titanium Scaffolds were produced by using a rapid prototyping technique. These scaffolds were either coated or not with a calcium phosphate coating via an eletrodeposition method. Rat bone marrow mesenchymal stem cells were cultured on the scaffolds at a density of 106 cells/scaffold for a period of 3 days. Cell proliferation was measured by using the Alamar Blue assay. The scaffolds were observed by SEM and polarized light microscopy. Constructs were then implanted subcutaneously for 4 weeks in syngenic rats. Cells proliferated well after seeding. After subcutaneous implantation, histology and SEM revealed the presence of uniform coatings as well as Ca and P deposits in the non-coated scaffolds suggesting mineralization.

Abstract: Two porous titanium implants with interconnected pore size of 800 and 1200 m in diameter, were fabricated by a rapid prototyping method. Their dimensions and structure accomplished the expected design with accuracy and reproducibility. The porosity of titanium was around 60%. The compressive strength and Young’s modulus were comparable to those of cortical bone with values around 80 MPa and 2.7 GPa, respectively. The implants were implanted bilaterally in the femoral epiphysis of 12 New Zealand Rabbits. After 3 and 8 weeks, abundant bone formation was found in the titanium porous structure. This work demonstrates that macroporous titanium with controlled shape and porosity is a good candidate for orthopaedic and maxilofacial applications.