Papers by Author: Tian Tian Meng

Abstract: Purpose: To search for the change of fibrinolytic activity of different forms of nattokinase when they were treated in human digestive juice, artifical gastric and intestinal juice differently, and to lay the foundation of a proper pharmaceutical dosage form of nattokinase. To provide direct evidences that Nattokinase products can be administered orally and to screen a suitable dosage form for oral administration of nattokinase.Methods: The non-crushed sample was prepared through the solid fermentation of soybean meal at 37°C for 36 h, and evaporation at 70°C for 8 h, The fine and ultra-fine powder were gotten from the non-crushed sample by the micronizer and ultra micronizer. Nattokinase extract was the supernatant liquid of 0.9% saline of non-crush samples. The nattokinase’s fibrinolytic activity of non-crushed sample, fine powder, ultra-fine powder and extract treated in artifical and human digestive were measured by the Fibrin plate method. Results: Three kind of solid form samples still remain about 80% fibrinolytic activity in artificial gastric juice for 4 h, and the fibrinolytic activity of nattokinase extract is 48.7% at the end of treatment. Treated in artificial gastric juice for 4 hours, then handled in artificial intestinal fluid for 4 h, the final fibrinolytic activity of fine and ultra fine powder is 47.0% and 51.0%. The fibrinolytic activity of extract is 79.8% at the end of treatment in human digestive juice for 4 h. At the beginning of treatment in human digestive juice the enzyme activity of fine and ultra fine powder is 130.8U/mL and 132.1U/mL, however, after treatment for 4 h the enzyme activity is 158.7U/mL and159.6U/mL, which is near to the total enzyme activity of sample. Conclusion: Nattokinase products can be administered orally, and solid preparations of nattokinase are more suitable for oral route. The optimum dosage form for oral administration of nattokinase is enteric-coated capsules containing ultra fine powder.

Abstract: To prepare out a novel preparation of liuweidihuang which effectively preserves Paeonol, Ursolic acid and Polysaccharides, and the preparation process should be scientific, lower-price and simple. The microscopic characteristics and its physicochemical properties of super-micro-particle of liuweidihuang from above process were also discussed. Methods According to the therapeutic and physicochemical properties of materials in liuweidihuang, the processed technology is as follow: Ripe rehmannia glutinosa,Tuckahoe and Alisma digitalis were boiled for 4.5h., the root bark of the peony tree(Paeonia suffruticosa), dogberry and yam were smashed to nanoparticles. Then select the reasonable method to drying the mixture. The microscopic shape characteristics of the before- and after- broken particles was compared. Paeonol content and dissolution were determined by HPLC. The stability and fluidity of liuweidihuang nanoparticles were examined by precipitation and funnel way. Results The approach of prepared liuweidihuang nano-microcapsules is scientific, lower-price and simple. The average size of processed super-micro-particle of liuweidihuang is 400±46nm. The hardness of the table is 9.7kg/cm2. The thickness of the table is 0.6cm. The friability of the tablet is ＜1%. Each tested item complied with standards of troches of Chinese medicine, and it is beneficial to industrial production for Chinese medicine.. Conclusion The nano-microcapsule is dispersive, desiccative, mobilizable, stable and three times concentrated than traditional pills of Liuweidihuang. It also can be used to produce different forms of drugs in the market.

Abstract: Objective In order to increase the Paeonol dissolution and content, cortex moutan were smashed into nanoparticles, and the dissolution and content were compared by microscopy before and after super-micro-particle pulverization. Methods Super-micro-particle pulverization and general grinding were used to broke Cortex moutan into particles. The microscopic morphous characteristics of the before- and after- ultra-disintegration particles were compared by microscopy. Methods of HPLC was used to determine the content and dissolution of Paeonol with different grinding conditions. Methods of precipitation and funnel way were used to examine the stability and fluidity of cortex moutan nano-particles. Results Cortex moutan powder after super-micro-particle pulverization appears sphere or like-sphere, and its average size is 200nm~300nm. After the superfine grinding Paeonol dissolution increases 76.19% in comparison with without nano pulverization. The nanoparticle rest angle is θ=33°.The precipitation ratio of Cortex moutan powder with general grinding is 0.28 at 24h, and the precipitation ratio of its nano-power has been to 0.98 at 60min. Conclusion Paeonol dissolution, stability and fluidity of Cortex moutan nanoparticles were improved greatly and this nanoparticles is beneficial to industrial production for traditional Chinese medicine.