Papers by Author: Yao Xing Xu

Abstract: In this paper, the properties of chitosan nanoparticles and bovine serum albumin-loaded chitosan nanoparticles prepared with sodium tripolyphosphate at different pH values were discussed. Interactions in chitosan nanoparticles and bovine serum albumin-loaded chitosan nanoparticles were analyzed by Fourier transform infrared spectroscopy and differential scanning calorimetry. The results indicated that there were more complicated interactions in chitosan nanoparticles prepared at different pH values. When the pH values were lower or higher than isoelectric point value of target protein (bovine serum albumin), the big different interactions occurred in chitosan nanoparticles. These differences also would result in different releasing properties.

Abstract: In this paper, the properties of chitosan (CS) nanoparticles (CS NPs) and Bovine Serum Albumin (BSA) loaded chitosan nanoparticles (BSA-loaded CS NPs) prepared with sodium tripolyphosphate (TPP) at different pH values were discussed. In addition, BSA encapsulation efficiency, zeta potentials and size of CS NPs and BSA-loaded CS NPs were characterized. The results indicated that sizes of CS NPs presented “V” change along with increase of pH values, while the size of BSA-loaded CS NPs showed “U” shape change. BSA encapsulation efficiency reached the biggest value when the pH value was 5.5. Zeta potentials of CS NPs and BSA-loaded CS NPs kept going down when the pH values were increasing, and positive potential of CS-loaded BSA CS NPs became higher than that of CS NPS when the pH value was more than 5.5. BSA-loaded CS NPs prepared at pH 5.5 exhibited regular and close to spherical in shape under transmission electron microscopy (TEM) sight.