Papers by Keyword: Antiproliferation

Abstract: Sirolimus-loaded poly (lactic acid-co-glycol acid) (PLGA) films were prepared by a casting method. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was performed to determine the optimal concentration of sirolimus for vascular smooth muscle cell (VSMC) inhibition effect. Based on the results of MTT, three samples of different weight percent loaded-sirolimus (2wt%, 6wt%, 15wt%) PLGA films were selected. The release of lactate dehydrogenase (LDH) was used to evaluate the immediate toxicity of the sirolimus-loaded PLGA films and it was shown that the three different concentrations of sirolimus-loaded films have low toxicity. Alamar blue results indicate that the sirolimus-loaded films have better antiproliferation effect than 316 stainless steel (SS). The higher the sirolimus concentration in the film is, the better is the antiproliferation effect. These films may be used for stent coating to inhibit in-stent restenosis induced by VSMC proliferation.

Abstract: The objective of this study is to investigate whether the PEGylated conjugated linoleic acid (PCLA) as an anti-cancer prodrug can have favorable stability, biological activity, and prevention of proliferation in MCF-7 breast cancer cells for anti-cancer when compared with conjugated linoleic acid (CLA) itself. The CLA was simply coupled to poly(ethylene glycol) (PEG) at melting state without solvent or catalyst through ester linkage between carboxylic group of CLA and hydroxyl one of PEG. The results showed that the half life of PCLA was 55h in cell culture medium at pH 7.4 and 37°C. Apoptosis of MCF-7 breast cancer cells were induced by not only CLA- but PCLA-treatment with increasing concentrations whereas PCLA increased cell viability when compared with CLA itself. These results indicate that the PCLA is a more stable and valuable prodrug in that it has good stability and inhibition of cancer cell proliferation.

Abstract: Rapamycin/curcumin co-loaded and rapamycin-loaded poly(lactide-co-glycolide)(PLGA) films were prepared by a casting method. The components of different drug-loaded films were analyzed by Fourier transform infrared spectroscopy (FTIR), and the major peaks of rapacymin and curcumin were both observed in these films. Compared to the rapamycin-loaded film, the results of in vitro platelet adhesion tests show that the number of adhered platelets reduce, and few aggregated and activated platelets are observed. The APTTs (activated partial thromboplastin time) of rapamycin/curcumin co-loaded films is nearly 6 seconds longer than for the rapamycin-loaded PLGA films. All results indicate that curcumin may suppress thrombosis activated by rapamycinloaded film, and improve the anticoagulative property. The results of alamar blue indicate that the rapamycin/curcumin co-loaded film has better antiproliferation effect than 316 stainless steel (SS).