Papers by Keyword: Bilayer Scaffolds

Abstract: When the articular cartilage defect accompanies with the subchondral bone defect, using bilayer scaffolds which can integrate with surrounding host cartilage and bone tissue respectively as the tissue engineering scaffolds will be conducive to the repair of tissue defects. This paper reports a new method for preparing bilayer scaffolds. Firstly, hydroxyapatite (HA)/silk fibroin(SF) composite porous materials which have high porosity were prepared by a isostatic compaction molding method, then it was fully immersed in silk fibroin solution, and finally SF/HA bilayer scaffolds were obtained by freeze-drying. The structure of the bilayer scaffolds were investigated through scanning electron microscopy, X-ray diffraction, Fourier transform-infrared spectroscopy, electron excitation spectroscopy and electron microprobe. The results indicated that the upper layer of SF/HA bilayer scaffolds is porous SF component, the under layer is the porous HA/SF composite component and the interface of the two layer is closely connected. Furthermore, mesenchymal stem cells from mouse bone marrow were seeded into the bilayer scaffolds and the results showed that the cells had a well adhesion and growth after culturing for 3 days.

Abstract: In this study, it is shown that it is possible to develop 3D-porous bilayer hydroxyapatite/chitosan scaffolds by means of combining a sintering and a freeze-drying technique. Scanning electron microscopy (SEM/EDS) studies revealed that the scaffolds possess a well-defined orientation and anisotropic porosity, with pore size ranging between 50-350 µm. Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) patterns evidenced the formation of crystalline hydroxyapatite. Moreover, the compression tests revealed that these scaffolds have adequate mechanical properties for being used in tissue engineering of osteochondral defects.