Papers by Keyword: Confocal

Abstract: This paper presents physical characteristic of Rhodococcus zopfii through the use of Environment Scanning Electron Microscopy. This bacterium is very significant in enhancing degradation of polycyclic aromatic hydrocarbons (PAHs) in the presence of natural biofilm in waste water treatment system. Rhodococcus Zopfii was isolated from municipal sludge at Universiti Teknologi MARA. The average size of Rhodococcus Zopfii was measured in the range of 1.1–2.85 μm lengths and 0.55-0.80 μm diameters. The colour of this bacterium was slightly pink on agar plate and it had rod shaped. Experimental results obtained from confocal laser scanning microscopy showed that this bacterium can easily attach on the surface of biofilm. The experimental results provide a scientific knowledge of physical characteristic of Rhodococcus Zopfii isolated from municipal sludge to grow on natural biofilm in wastewater treatment system.

Abstract: With the wide application of thin film technology and optoelectronic devices, optical films have been widely used in the development and production of weapons and special devices for basic research. Faced with constantly updated status quo and development of optical thin film precision measurement of various parameters on a higher requirements, the film thickness is one of the key parameters in film design and manufacturing process. Particularly with the rapid development of nanoscale thin-film technology, the film thickness becomes a hot issue in the research field. For solid film thickness, the main measuring means are screw micrometer, microscopy, interferometry and polarization. Conventional Michelson interferometer can measure thin film thickness which is transparent and known, and the devices with sensors performance better.

Abstract: The mammalian target of rapamycin（mTOR）is a kind of Ser/Thr kinase in mammalian cells. It can recruit and integrate input signals from nutrients, growth factors, energy and environmental stress to regulate cell growth and proliferation via different cellular processes. This study uses the fetal fibroblasts of Inner Mongolia Cashmere goat (Capra hircas) to prove that the mTOR plays a critical role in formation of the cell cytoskeleton structure. The mTOR kinase activity was inhibited in Inner Mongolia Cashmere goat fetal fibroblasts (GFb) after treatment with CCI-779 (temsirolimus), an mTOR specific inhibitor for 48 h. The results showed that GFb cells were sensitive to CCI-779. GFb cells morphology and its cytoskeleton structure changed under confocal laser scanning microscopy stained with the Fluorescent phalloidin (50µg/ml, Phalloidin-FITC5282) which combines with F-actin. In summary, mTOR signaling pathway was proved to be functional in GFb cells and acts as a key regulator to form cell structure. The Morphological results indicated that perhaps the synthesis of microfilament or organization of cytoskeleton was disrupted in GFb cells when mTOR was inhibited.

Abstract: A novel multi-channel DNA fragment analysis system is presented，which is based on single PMT confocal fluorescence detection and optical scanning adopting an f-theta lens. Capillary electrophoresis experiments were executed in the system for DNA marker pBR322/Hae III. Signal was processed with wavelet denoising. The system’s limit of detection (dsDNA with the probe of Thiazole Orange, TO) was evaluated to be 1.1841×10-11mol/L. Its working noise is much lower compared with that based on mechanical scanning one, and the stability and detecting sensitivity is high. The system was expected to be applied to both capillary array and microchip electrophoresis detection based on laser-induced fluorescence.

Abstract: In this paper, the modulation transfer function with aberration of a multichannel laser induced fluorescence analyzer is presented. Based on an f-theta lens, the confocal system was used both to transform the exciting laser and to collect the fluorescence emitted from sample. The total MTF is the combination of that of both systems. A simple method for the numerical calculation of that was also given. From the calculating result we could conclude that the aberration had poor impact on the MTF of a confocal multichannel fluorescence analyzer and the imaging performance was uniform in the full field.

Abstract: Laser scanning confocal microscopy (LSCM) is a microscopic technique which allows for height discrimination. The ability to gather 3D data, along with adequate resolution (around 400 nm), makes the technique suitable for fractography; however, its applications in this area are not sufficiently explored. In this work, LSCM and SEM are applied to the study of fracture surfaces in sapphire and ruby fibers submitted to tensile stress in high-temperature conditions. The obtained qualitative and quantitative information demonstrates the validity of LSCM as a fractographical technique, allowing for clear identification of fractographical features and providing novel insight in the phenomenon of subcritical crack growth (SCG).