Papers by Keyword: Conjugated Linoleic Acid

Abstract: Conjugated linoleic acid (CLA) was prepared by alkali isomerization using walnut oil as raw materials, KOH as catalyst and propylene glycol as solvent. The kinetics of alkali isomerization of linoleic acid from walnut oil converted into CLA was experimentally studied under the reaction conditions of 393~453 K,and which the initial mass ratio of propylene glycol, walnut oil and KOH was 3:2:1. The mechanism of alkali isomerization was proposed, and the mechanism model was put forward through theoretical derivation.The rate constants were determined, the mechanism model was verified,and the kinetics parameters were obtained.The results showed that alkali isomerization reaction was first order the concentration of linoleic acid, which the apparent activation energy was 67.22 kJ·mol^-1 and the pre-exponential constant was 8.963×10⁵min^-1.

Abstract: The aim of this study was to select CLA producing bacteria from fermented diary products and human faeces, then evaluate their resistance to simulated gastrointestinal stress and adhesiveness to human intestinal epithelial cells according typically assess methods. Additionally, hydrophobicity, resistance to opportunistic pathogens and phenol ability were analysed. Results showed that three L. paracasei subp. Paracasei (F0721, IN 5.22 and Q9), two Lactobacillus acidophilus (F0221 and M6) and one Lactobacillus plantarum (G14) exert the ability to product conjugated linoleic aicd ranging from 77.21 to 111.59 μg/ml in the presence of 0.5 mg/ml linoleic acid (LA) in MRSc broth under anaerobic conditions at 37°C for 40 h. Strain F0721, IN5.22 and G14 showed higher resistance to gastric acid than other strains at pH 2.0, F0721 and IN5.22 showed higher tolerance ability at pH 3.0. F0221 showed the highest bile salt tolerance than other strains at 0.15%, 0.3% and 0.5% bile concentrations, whereas highest sensitivity to the bile stress was found for IN5.22. F0221, IN5.22 and G14 strains showed the highest levels of adherences (range from 106 to 129 cfu/100 cells), and intestine origin bacteria (F0221, F0721 and IN5.22) exhibited higher hydrophobicity values and resistant to phenol ability as compared to the dairy products.

Abstract: The objective of this study is to investigate whether the PEGylated conjugated linoleic acid (PCLA) as an anti-cancer prodrug can have favorable stability, biological activity, and prevention of proliferation in MCF-7 breast cancer cells for anti-cancer when compared with conjugated linoleic acid (CLA) itself. The CLA was simply coupled to poly(ethylene glycol) (PEG) at melting state without solvent or catalyst through ester linkage between carboxylic group of CLA and hydroxyl one of PEG. The results showed that the half life of PCLA was 55h in cell culture medium at pH 7.4 and 37°C. Apoptosis of MCF-7 breast cancer cells were induced by not only CLA- but PCLA-treatment with increasing concentrations whereas PCLA increased cell viability when compared with CLA itself. These results indicate that the PCLA is a more stable and valuable prodrug in that it has good stability and inhibition of cancer cell proliferation.