Papers by Keyword: Fluorescence Ratio

Abstract: A new effective imaging detecting system has been used to determinate of intracellular calcium. The fluorescence Image acquisition and analysis was realized with fluorescence imaging detecting system. A designed microfluidic chip has been built. Cell fostering, staining and other operations can be implemented on it. Based on it, a new detecting means was developed for fluorometric measurement. The concentration of intracellular calcium ion can been calculated by using the fluorescence ratio method. A series of experiments were performed by using the detecting system. The experimental results show the ratio of fluorescence intensity was positively correlated to the raising temperature and enhancing shear stress.

Abstract: Chemical mechanical polishing has emerged recently as an indispensable processing technique in large scale integration. In chemical and mechanical polishing process, chemical and mechanical principle is the vital factor in the removal process. Little is known about what is occurring beneath a wafer during Chemical Mechanical Polishing (CMP) processes. The paper provides a LIF technology to visualize the fluid flow between the wafer and pad. In this paper, the experiment setup is built. And then, the images of fluorescence intensity excited by LIF have been obtained from CCD. Finally, the relationships between pH, temperature, laser power, film thickness and fluorescence intensity excited by LIF (Laser induced Fluorescence) are studied.