Papers by Keyword: Fructus tribuli

Abstract: Effect of hot water extract (HWE) from Fructus tribuli on growth of Lactobacillus casei, Lactobacillus reuteri, Lactobacillus rhamnosus and Lactobacillus bulgaricus with material properties was studied by measuring optical density at 600nm (OD₆₀₀) and pH using MRS media as the control. The addition of HWE (v/v) was 1%, 2%, 3%, 4% and 5%. Results were as follows: addition of HWE has the significant inhibition on growth of Lactobacillus reuteri and Lactobacillus bulgaricus and the significant promotion on growth of Lactobacillus casei and Lactobacillus rhamnosus. The optimum concentration of HWE in MRS media was 2% for Lactobacillus casei and 3% for Lactobacillus rhamnosus, respectively.

Abstract: Effect of steroidal saponins and flavonoid from Fructus tribuli on growth of Lactobacillus brevis was studied by measuring the optical density at 600nm and pH using De Man, Rogosa and Sharpe (MRS) broth as the control. The addition (mg/ml) of steroidal saponins was 0.10, 0.20, 0.30, 0.40 and 0.50, and the addition (mg/ml) of flavonoid was 0.10, 0.15, 0.20, 0.25 and 0.30. Results were as follows: The additions of steroidal saponins and flavonoid had a significant promotion on the growth of Lactobacillus brevis. The optimum concentrations of steroidal saponins and flavonoid were 0.20mg/ml and 0.15mg/ml respectively.

Abstract: Effect of the extract of Fructus tribuli (EFT) on growth of Lactobacillus acidophilus LA04, LA05, LA06 and LA09 was studied by measuring optical density at 600nm (OD600) and pH using MRS culture media as the control. The addition of EFT (v/v) was 1%, 2%, 3%, 4% and 5%. Results were as follows: addition of EFT could promote the growth of Lactobacillus acidophilus LA09, The optimum concentration of EFT in MRS media was 1% at incubation 24h, but addition of EFT on growth of Lactobacillus acidophilus LA04, LA05 and LA06 has no significant effect.

Abstract: Effect of steroidal saponins fromFructus tribuli on growth of Bifidobacterium bifidum BB01, BB03 and Lactobacillus acidophilus LA09 was studied by measuring optical density at 600nm (OD600) and pH using MRS media as the control. The concentration of steroidal saponins (w/v) added in was 0.05%, 0.10%, 0.15%, 0.20% and 0.25%. Results were as follows: addition of steroidal saponins could improve the buffer ability of MRS and promote the growth of Bifidobacterium bifidum BB01 and BB03. The optimum concentration of nutrient supplement in MRS media was 0.20% at incubation 24h for Bifidobacterium bifidum BB01 and 0.25% at incubation 24h for Bifidobacterium bifidum BB03. Addition of steroidal saponins on Lactobacillus acidophilus LA09 didn’t play an obvious role in promoting growth when it was in high level.