Papers by Keyword: Hemolysis Test

Abstract: nanomagnesium hydroxide (Mg (OH)₂) is promising potential material for biomedical applications, such as nanomedicine. However, little is known about the hemolysis of nanostructured Mg (OH)_2. In the present investigation, Mg (OH)₂ nanoparticles synthesized by precipitation technique using magnesium nitrate and sodium hydroxide. The phase structure and morphology of the Mg (OH)₂ nanoparticles are characterized by X-ray powder diffraction (XRD), selected area electronic diffraction (SAED) and transmission electron microscopy (TEM) respectively. Hemolytic activities in vitro are carried out on the synthesized nanoMg (OH)₂ samples and the studies reveal that Mg (OH)₂ nanoparticles have mild activity against erythrocytes.

Abstract: Objective:To evaluate the blood compatibility of a central venous catheter in order to provide evidence for the safety of medical devices. Methods:The indexes of coagulation including thrombin time(TT), prothrombin time(PT), activated partial thromboplastin time (APTT) and fibrinogen (Fg) concentration, as well as the indexes of platelet function parameters including TXB2 and platelet count were measured, hemolysis test was conducted as well to detect the hemolysis rate of the sample. Results:There was no significant difference between the samples and negative controls on indexes of coagulation and platelet function (p>0.05), hemolysis test showed that the hemolysis rate of the sample was 1.35% (less than 5%), which was coincident with the criteria of the medical devices. Conclusion:The test sample of central venous catheter has good blood compatibility.

Abstract: Objective: To evaluate the biological safety of a colored zirconia ceramic regarding its hemolytic activity and short-term systemic toxicity. Methods: Fresh anti-coagulating rabbit blood was mixed with test materials and the hemolytic activities detected spectrophotometrically. Suspensions of the ceramic were fed to rats for one week while monitoring food consumption and relative growth rate and with subsequent pathology examinations of vital organs and tissues. Results: The hemolytic activity of the ceramics was <5%, and rats in all groups showed weekly relative growth rates of food utilization and body weight with no statistically significant differences. There were also no pathological changes observed among the various examined organs between the experimental and control groups. Conclusions: This colored ceramic showed good biocompatibility in terms of hemolytic activity and short-term systemic toxicity.

Abstract: Objective: to evaluate the biological safety of a colored alumina/glass composite in terms of cytotoxicity and hemolytic ability. Methods: the cytotoxicity of an alumina/glass composite was evaluated with molecular filtration method. Cytotoxicity by spectrophotometric responses of test material and responses of a fresh rabbit red blood anticoagulant solution was determined by spectrophotometer. Results: cell-coated filter paper in contact with the test material showed the appearance of intracellular blue dye as the same staining density as in control samples, leading to a toxicity rating of 0. Colored alumina/glass composite assayed at <5% hemolysis. Conclusion: colored alumina/glass composite materials showed low cytotoxicity and non-acute hemolytic rates, representing overall good biological safety under these conditions.

Abstract: Hydroxyapatite (HA) powder was synthesized by a sol-gel method with Ca(OH)2 and H3PO4 as reactants. The HA granules were then coated with TiH2 powder using a mechanical mixing method. The HA-TiH2 material system produced HA-Ti composites after hot-pressing at 1050°C. The HA-Ti composites are mainly composed of HA and Ti, with small amounts of Ca2P2O7 and Ca3(PO4)2 phases. Fracture toughness and bending strength are 2.4 MPa·m1/2 and 54.3 MPa, respectively for the HA-20vol%Ti composite, higher than those of the pure HA ceramic. The improvement in properties is because of the unique 3D network structure of Ti, which is an ideal reinforcement structure for the weak and brittle HA. According to ISO/TR 7405-1984, hemolysis test was performed to evaluate the blood compatibility of the material. The results show that the hemolysis rate of the HA-20vol%Ti composite is 0.56%. Relative growth rates (RGR) of L-929 cells soaked after 6 days in the HA-20vol%Ti group, pure Ti group, black group and pure Pb group were 132%, 100%, 90% and 6% respectively, while the level of cytotoxicity was grade 0 in HA-Ti composite group. These results imply that the HA-20vol%Ti composite has good biocompatibility and bioactivity.