Papers by Keyword: HepG2

Abstract: Photothermal-temperature responsive [Ag nanoparticles-hollow mesoporous silica nanospheres–poly (N-isopropyl acrylamide-acrylic acid)] (Ag@HMSN@PNIPAM-AA) nanoparticles were designed and prepared, and the combination of Ag nanoparticles (AgNPs) and [poly (N-isopropyl acrylamide-acrylic acid)] (PNIPAM-AA) was used as a switch of the photothermal-temperature effect to control drug release. The results of cell culture in vitro showed that [mesoporous silica nanospheres–poly (N-isopropyl acrylamide-acrylic acid)] (MSN@PNIPAM-AA) and Ag@HMSN@PNIPAM-AA nanoparticles had good biocompatibility and less cytotoxicity, and Ag@HMSN@PNIPAM-AA nanoparticles had higher cell inhibition under 808 nm near-infrared light. The combination of near-infrared light and doxorubicin showed higher drug release efficiency and a stronger inhibitory effect on HepG2 cell growth. These characteristics indicate that Ag@HMSN@PNIPAM-AA nanoparticles have great potential for treatment. This study also proved the universal applicability of Ag@HMSN@PNIPAM-AA nanoparticles. Different model drugs and nanoparticles can play different roles and have development potential.

Abstract: The cytotoxicity of Desacetylcinobufotalin (DEBF) and apoptosis induced by DEBF was measured. Additionally the mechanism of Apoptosis induced by DEBF was studied through Western blot. The results show DEBF displayed the marked inhibition effect to HepG2 cells and the IC₅₀ value is 0.0279μmol/ml. The expression of Bax was significantly increased and the expression of Bcl-2 was markedly decreased, compared to the control. The data suggest DEBF had significant antitumor activity through induction apoptosis via mitochondria pathway.

Abstract: To investigate the mechanism on Capparis spionosa L polysaccharide(CSPS) inducing apoptosis in HepG2 human hepatoma cell. MTT was ddopted to determine if CSPS had cytotoxic effect on HepG2. Morphology of HepG2 changed with dosages of CSPS was detected by laser confocal scanning microscope. Flow cytometry(FCM) was used to detect the apoptosis by PI labeling method. Calcium, mitochondrial membrane potential, Bcl-2/Bax of HepG2 cells were detected by laser confocal scanning microscope. The result of MTT showed that CSPS could inhibit the growth of HepG2 significantly. HepG2 cells were shrinkage, fragmentation, appearance of apoptotic bodies by laser confocal scanning microscope. HepG2 cell apoptosis rate was increased gradually with dosage by FCM. Calcium concentration, mitochondrial membrane potential, Bcl-2 protein of HepG2 were decreased, Bax protein content of HepG2 was increased by laser confocal scanning microscope. CSPS induced HepG2 apoptosis by controlling Bax/Bcl-2 in Ca²⁺ path.

Abstract: Porous & rubbery organic-inorganic hybrids were synthesized from tetraethoxysilane (TEOS) and polydimethylsiloxane (PDMS) through a sol-gel route using sieved sucrose granules as a porogen. The porous hybrids with a high content of PDMS behaved like polymer sponge. The porosity was over 90% irrespective of the hybrid composition and the pore diameter ranged from 100 to 500 μm. In the three-dimensional cell culture, mammalian cells were well cultured in the porous hybrids. The present results indicate that the hybrids may be a promising scaffold for developing such functional culture methods.