Papers by Keyword: Liquid Cell

Abstract: This paper describes a novel device, a wet cell, used for observation in the wet environment inside the scanning electron microscope (SEM). A Wet cell is composed of three counterparts, which are flow control system, thermal system and Wet cell body. The flow system provides continuous buffer liquid to wet cell and maintains cells living at the vacuum environment. It can control different flowrates and switch to different fluidics. Additionally, the thermal system can control the temperature inside the wet cell and maintain at 37 °C. It also offers preheating thermal module to lower the fluctuated of wet cell inside temperature. The wet cell body contains a silicon nitride film based observation window and provides a wet environment for observation between vacuum and anti-vacuum status. In this paper, we introduce the structure and experimental data of flow control and thermal system in Wet cell to let SEM easily provide lots of live imaging information such as dynamics of nano-materials and biological samples in the liquid state.

Abstract: This paper describes the development and validation of the liquid cell smears device for early cancer detection. In this study, the liquid cell smears device with the automated production of cell slides for Liquid-based Cytology Test has been developed. To validate the Liquid cell smear device, we used each 100 samples related to cervix, sputum, urine, body fluids, and thyroid. Experimental conditions were divided into five, which were the distance of the cylinder, the moving party's ascent and descent distance, and time to stop the smears. After staining, their verification was conducted through cytologic and histologic diagnosis. As a result, we found the optimal conditions to produce slide such as cervix(GYN) at the condition 2, sputum(SPUTUM) at the condition 4, urine(URINE) at the condition 5, body fluids(BODY FLUID) at the condition 1, and thyroid(FNA) at the condition 3. Therefore, it is suggested to be possible as cytologic and histologic diagnosis.