Papers by Keyword: MS/MS

Abstract: We proposed a MALDI mass spectrometry based approach to characterize walnut allergens. The strategy is based on the extraction of hydro-soluble tissue proteins followed by protein fractionation and mass-spectrometry analysis. Linear MALDI was adopted to evaluate the intact protein mass information and the presence of glycoproteins.

Abstract: A rapid method for analysis of the main components of Radix Glycyrrhizae (RG) was developed using ultra-performanceliquid chromatography with quadrupole-time-of-flight mass spectrometry (UPLC/Q-TOF-MS). Previous study demonstrated that RG had antivirus, antioxidant，anti-tumor and other bioactivities. The separation analysis was performed on Waters Acquity UPLC system and the accurate mass of molecules and their fragment ions were determined by Q-TOF MS. Fourty compounds including triterpenoid saponins, flavonoid glycosides and flavonoid aglycones,coumarins, were identified and tentatively deduced on the basis of their element compositions, MS/MS data and relevant literatures. The results demonstrated that triterpenoid saponins, flavonoid glycosides and flavonoid aglycones were the main constituents of RG. Furthermore, an effective and rapid pattern was established allowing for comprehensive and systematic characterization of the complex samples.

Abstract: The suitability of high performance liquid chromatography (HPLC) for the direct determination of the concentration of complexing agents for single chemistry cleaning is demonstrated. HPLC, coupled to a mass spectrometric detector (HPLC-MS) and two-dimensional mass spectrometry (MS/MS) have been applied for the investigation of the reactions involved in the decomposition of the complexing agents. The techniques described are useful for determining the stability of organic additives in wet chemical cleaning baths.