Papers by Keyword: MTT

Abstract: In recent years, the technology of dental implants has advanced a lot and this has become one of the main reasons for dentists to refer to implants immediately after tooth extraction. Evaluation of cell survival in implantology studies to determine cell sensitivity determines the outcome of treatment. This study aimed to investigate the bone integration properties as well as the cytotoxicity between the implant surface and the jaw bone. In this study, cytotoxicity test was used to evaluate the toxicity and viability of sandblasted large grit acid etched, and sandblasted large grit acid etched active surfaces in 3A brand dental implants with G292 osteoblastic cells and human gingival fibroblasts cells are discussed. This operation was performed using a laboratory incubator of the German company MEMMERT for 24 hours, by neubauer lam cells counting for one hundred thousand cultured cells in each test at a temperature of 37 °C, a pressure of 1 atmosphere and 90% humidity. Based on the scanning electron microscopy images and the cytotoxicity test results, it can be seen that the bone graft of the implant, with the sandblasted large grit acid etched active surface treatment, is much better and also one week faster than the implant with the sandblasted large grit acid etched one. However, the viability of the implant with the sandblasted large grit acid etched active surface treatment for both G292 osteoblastic cells and human gingival fibroblasts cells samples was equal to 98.4% and 97.3%, respectively, and is lower than the sandblasted large grit acid etched surface treatment. The results show that the viability of the sandblasted large grit acid etched implant is about 1.5 to 2% higher than the sandblasted large grit acid etched active one, but the surface integrity of sandblasted large grit acid etched active is better than sandblasted large grit acid etched in all samples, and the treatment process is reduced by one week.

Abstract: Objective: The aim of this study was to evaluate the cytotoxic effects of three commercial light-cured orthodontic adhesives.Materials and methods: The potential cytotoxic effects of three types of orthodontic adhesives, Grengloo, Green Glue, and Transbond XT, were tested on L929 cell culture. The cell line was grown in 96-well tissue culture plates (1x10⁵ cells/mm³). Thin resin discs weighing 0.4, 0.6, 0.8, 0.8, and 0.8 gram of each material were prepared and aged for 1, 3, 6, 8, and 10 days, respectively, in Minimum Essential Medium (MEM) at 37°C with 5% CO₂ at 100% humidity. Cell viability was assessed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay according to ISO 10993-5: 2009 (E). The differences among the groups was statically analyzed by independent paired t-test (α = 0.05).Results: After 1 day of storage, all adhesive systems showed cytotoxic effects. However, ageing tended to considerably reduce the cytotoxicity of Green Glue. Grengloo was essentially non-cytotoxic day 3 onwards, while Green Glue and Transbond XT exhibited potential cytotoxicity at all times of the experiment. Conclusion: All tested light-cured orthodontic adhesives had cytotoxic potential during the first day. Grengloo had the highest cell viability, whereas, Green Glue had the lowest.

Abstract: Indole and its derivatives extensively exist in nature, which showed a wide range of biological activities. Recently, there is a growing tendency to develop some new methods for the synthesis of novel indole derivatives and to study their biological properties. In this paper, we would like to report the design, synthesis and biological evaluation of a novel series of tricyclic indoles by employing Pictet-Spengler reaction as key step. All the target compounds were synthesized in four steps in 68-78% overall yield, which were characterized by ¹H-NMR spectrum. All this new compounds were tested in an MTT assay on HepG2 and K562 and HT-29. Two of them showed tumor cell growth inhibitory good activity.

Abstract: Indolone is a kind of natural product which was widely existed in the animal bodies and plants, which possess varieties of biological activities, such as anti-bacterial, anti-tumor, anti-aging and anti-anxiety activities. As we all known that many sprio compounds which containing hetero atom such as O, N, S have good biological activities. In this paper, a series of spiro indolone derivatives was design with the combination of indolone and sprio scaffold, 6 novel target compounds were synthesized in 3 or 4 steps in 6%-17% overall yield, which were characterized by ¹H NMR and their biological activities on tumor cells growth inhibition on human hepatoma cell HePG2 and human leukemia cell K562 by MTT method were evaluated.

Abstract: Supercritical CO₂ was used to extract effective compounds from pine pollen, MTT colorimetric method was applied to determine anti-cancer effect of effective compounds in pine pollen. Results indicated that extracts obtained at pressure ranges 0~30 MPa and 10~15 MPa had significant inhibition effect on the lung cancer cells 95D of pig (95D) and their IC50 value was 253.22 μg/mL and 252.19 μg/mL, respectively. Extracts attained at pressure ranging 0~10 MPa, 10~15 MPa and 0~30 MPa had significant inhibition effect on the stomach cancer cells SGC-7901 of human (SGC-7901), and their IC50 value was 248.35 μg/mL, 459.56 μg/mL and 301.20 μg/mL, individualy. Supercritical CO₂ extract of pine pollen had inhibition effects on the lung cancer cells 95D of pig and the s_{Subscript text}tomach cancer cells SGC-7901 of human, and the corresponding active compounds were different.

Abstract: A novel Na₂O-K₂O-CaO-MgO-SrO-B₂O₃-P₂O₅ borophosphate glass is prepared by melting method. The thermal properties have been examined by differential thermal analysis (DTA) and viscosity measurement. The bioactivity of the glass is measured via soaking the glass in the SBF solution at 37 °C for different time. The reaction of the glass in the SBF solution is characterized by XRD, FTIR and SEM. The results indicate that the carbonate hydroxyapatite deposit on the glass in less than 7 days. Cell attachment, spreading and proliferation on the glass is determined by MTT [3-(4, 5-Dimethylthiazol-2-yl)-2, 5-Diphenyltetrazolium Bromide] assay method using Human osteosarcoma MG-63 cells. The borophosphate glass fiber is also drawn and the tensile strength is measured. The results suggest the borophosphate glass has a good potential to be a scaffold material for bone tissue engineering.

Abstract: The cytotoxicity and anti-influenza virus (IFV) activity of calcium or zinc alginate fibers were investigated to explore the feasibility of them to be used as biomaterials. African Green Monkey kidney cell (Vero) and human cervical cancer cell (Hela) cultured with alginate fibres were used to screen cytotoxic effects. After 48 h, MTT (3-(4,5-dimethyl-2-thiazol)-2,5-diphenyl-2H tetrazolium bromide) assays were performed. Then cytotoxicity was evaluated with six grades according to cell relative growth rate (RGR). In anti-IFV activity assay, IFV were added to all fibers and the Vero cell survival were detected by MTT assays with calculating the percentage of protection. The cytotoxity of calcium alginate fibers on Vero were grade 0 or 1 in contrast to zinc alginate fibers which was grade 0. The cytotoxity of calcium or zinc alginate fibers on Hela were grade 0. Furthermore, partial calcium or zinc alginate fibers could promote Vero or Hela cell growth. In antiviral assay the highest percentage of protection of calcium alginate fibers was 34.42%, while that of zinc alginate fibers was 59.42%. The results showed that calcium or zinc alginate fibers had a good cellular biocompatibility and the large weight zinc alginate fibers had a better anti-IFV activity than calcium alginate fibers, which is potential for tissue engineering.

Abstract: Poly ε-caprolactone(PCL)/hydroxyapatite(HA) composite scaffolds were fabricated by particulate leaching and freeze drying routes with different HA content. Porosity was decreased with HA addition, while mean pore size was maintained at around porogen size regardless of HA content. Compressive modulus was increased with increasing HA content. In this study, the optimum content of HA was around 40% in weight against PCL to obtain the highest compressive modulus with keeping porosity above 85%. HA apparently enhanced proliferation of osteoblast-like MG63 cells in PCL/HA composite scaffolds. Typical adhesion, migration and aggregation procedure of MG63 cells were found on PCL, while spreading morphology only was found on HA even at the early stage of adhesion without migration or aggregation.

Abstract: Cytotoxicity test was essential for the pre-clinical evaluation of bioceramics. Proliferation assays such as MTT, XTT and WST-1 were commonly used for measuring biocompatibility. WST-1 was more convenient than MTT because of its water-solubility and storage condition. The calcium phosphate glass and β-TCP have been used for bone substitute, and some magnetic ferrites have been used for hyperthermic treatment. L929, mouse fibroblast cell, was the representative cell-line for in vitro biocompatibility test. The extracts of test samples were prepared by ISO10993-12:2002. The biocompatibilities of the extracts were measured by MTT and WST-1 assay and their pH were measured with pH meter. The cellular survival rate of CPG was the lowest and the results of the WST-1 test showed results similar to those of the MTT test. Thus, proliferation assays using WST-1 may be conveniently and routinely applicable to pre-clinical evaluation of bioceramics.