Papers by Keyword: Phosphorylation

Abstract: Loess with large thickness is widely distributed mainly in Gansu provinces of China. The kind of loess has special microstructure to induce lower dynamic strength and larger seismic deformation. It brings to difficult loess processing challenges for engineering building foundations or high way and railway constructions. The large deformation dangers could induce to serious seismic subsidence, watering deformation or loess landslip. There have been series problems of how to change its natural microstructure for engineering demanded which is major concerned in this paper. We put forward Phosphoric modified methods to treat loess problems. By changing different proportions of adding filling materials, such as Calcium carbonate, barite, talcum powder, and others auxiliaries including phosphoric acid and barium metaborate, found it get to be better effect. It’s dynamic deformation coefficients dropped lower than 2‰. After loading test, the methods prove to be greatly enhanced loading strength and changed its unreasonable microstructure. Because of no toxicity materials be adopted and produced during course, the method can be popularized and applied.

Abstract: P-ferrocene-SA/acetylferrocene-CS Bipolar Membrane (BPM) was prepared with phosphorylated agent as the cation exchange layer, and chitosan (CS) modified with acetylferrocene as the anion exchange layer. Zinc/Nickel alloy layer was placed on the surface of cation exchange layer to realize zero polar distance in the cathode chamber. The 2, 3, 5, 6-tetrachlopyridine (2,3,5,6-TCP) was electro-synthesized by reducing of pentachloropyridine(PCP). At the current density of 30mA·cm^-2, the current efficiency was 70.1% and the yield of 2, 3, 5, 6-TCP was up to 96%. Compared with the traditional method, the electro-generation technology greatly eliminated the environmental pollution.

Abstract: Based on the actual conditions during industrial production, this paper proposes an improvement method and solution to the typical grinding process model and derives a mathematical model of grinding process that’s suitable for industrial production. Sampling analysis and laboratory experiments of the production process were conducted, the derived model was tested, and the results show that particle size distribution curve obtained from the improved model is similar to the actual product, which means that the improved model can be applied to simulate industrial grinding process.

Abstract: Deoxyhypusine synthase (DHS) catalyzes the first step in the posttranslational synthesis of hypusine in the eukaryotic initiation factor 5A (eIF5A) precursor protein. As such, the phosphorylation of DHS by the protein kinase CK2 was investigated to define the role of DHS in the regulation of eIF5A in cells. The results showed that DHS was phosphorylated by CK2 in vivo as well as in vitro. The endogenous CK2 in HeLa cells and cell lysates was able to phosphorylate DHS and this modification was enhanced or decreased by the addition of CK2 effectors, such as polylysine, heparin, or poly (Glu, Tyr). A phosphoamino acid analysis of the enzyme revealed that the DHS was mainly phosphorylated into the Thr residue, with the remainder into the Ser residue. Therefore, it would appear that the phosphorylation of DHS was a CK2-dependent cellular event, thereby opening the path for possible regulation of the interaction with the eIF5A precursor for hypusine synthesis.