Papers by Keyword: Probiotics

Abstract: The objective of this study was to investigate the antioxidant capability and preventive effect of probiotics on high fat diet induced oxidative damage. Thirty male rats were randomly divided into three groups.The control group consumed a normal standard diet (5% fat, w:w), the high fat diet group received a high fat diet (20% fat, w:w), and probiotics group received a high fat diet supplemented with 2% probiotics. After 6 weeks, antioxidant capability of liver was determined and DNA fragmentation of liver cell was determined using a comet assay. The results showed that high fat diet could induce oxidative stress, shown as significant increases in lipid peroxidation and nitric oxide free radical, significant decrease in activities of superoxide dismutase and glutathione peroxidase, the percentage of DNA fragmentation was markedly increased, compared with control group. These alterations were significantly reversed in probiotics supplemented group and had no significant difference in antioxidant capability, lipid peroxidation and DNA damage compared with control group. Present observation indicated the probiotics had antioxidant property, which could scavenge free radical and enhance antioxidant defense system, and had the positive protective function on oxidative damage.

Abstract: Three Lactobacillus strains were screened on the basis of probiotic characteristics (i.e., resistance to low pH and bile salts, adhesion to the human gastrointestinal tract, inhibition of pathogenic strains). They further exhibited producing antimicrobial activities of non-acid molecule (s). In addition, antibacterial peptides were isolated and purified from the cell-free culture supernatants of these three probiotic strains. Based on TricineSDSPAGE, the antimicrobial peptide was approximately 10 kDa in size. After analyzing the sequence of the 16SrDNA regions of these three strains, they were identified as Lactobacillus crispatus Lactobacillus rhamnosus and Lactobacillus rhamnosua GG. Using an in vitro system simulating gastric transit, our findings indicated that the three probiotic strains had the ability to tolerate gastroenteric environment and the adhesive capacity to HT-29 cells. It was demonstrated that the probiotic strains inhibited subsequent adhesion of E. coli to the HT-29 cell. Among the selected strains, L. rhamnosus F1333 showed a high probiotic potential and could be used in health-promoting food products.

Abstract: Fermentation, a process traditionally known for the anaerobic conversion of sugar to carbon dioxide and alcohol by yeast, now refers to an industrial process of manufacturing a wide variety of metabolites and biomaterials by using microorganisms or mammalian cells in a controlled culture environment. Fermentation can be performed in batch mode, continuous mode or in a combinatory, fed-batch mode, depending on the product of interest. Fermentation technology has long been known for the production of various medically important products such as antibiotics, solvents such as ethanol, intermediary compounds such as citric acid, probiotics such as yoghurt etc. New generation fermentation products include anti-viral drugs, therapeutic recombinant proteins and DNA, and monoclonal antibodies. Apart from the drugs, fermentation is also used for the commercial production of materials required for the development of diagnostic kits, drug delivery vehicles and medical devices. Fermentation technology remains at the heart of rapidly growing biopharmaceutical industry today, which is expected to expand even more in the days ahead, in parallel with the progress in novel, targeted drug discovery.

Abstract: Effect of hot water extract (HWE) from Fructus tribuli on growth of Lactobacillus casei, Lactobacillus reuteri, Lactobacillus rhamnosus and Lactobacillus bulgaricus with material properties was studied by measuring optical density at 600nm (OD₆₀₀) and pH using MRS media as the control. The addition of HWE (v/v) was 1%, 2%, 3%, 4% and 5%. Results were as follows: addition of HWE has the significant inhibition on growth of Lactobacillus reuteri and Lactobacillus bulgaricus and the significant promotion on growth of Lactobacillus casei and Lactobacillus rhamnosus. The optimum concentration of HWE in MRS media was 2% for Lactobacillus casei and 3% for Lactobacillus rhamnosus, respectively.

Abstract: 120 crucian carp were divided into 4 groups. Each group included two repeats with each of 15 crucian carp, and was fed with feedstuff mixed probiotics concentrations at 0.0%, 0.2%, 0.4%, and 0.6%. The figure index and viscera index were measured, and the nutrient compositions were analyzed after 32 feeding days. As a result, the best probiotics concentration is 0.4% on the increase of the weight gain and the nutrient compositions.

Abstract: Effect of kanamycin sulfate and gentamicin on growth of Lactobacillus acidophilus, Bifidobacterium bifidum, Lactobacillus casei, Lactobacillus rhamnosus and Lactobacillus bulgaricus was studied by measuring the optical density at 600nm and pH using MRS broth as the control. The addition of kanamycin sulfate (%, w/v) was 0.0025, 0.005, 0.0075 and 0.01 and the addition of gentamicin(U/ml) was 48, 72, 144, 192 and 240. Results were as follows: addition of kanamycin sulfate at the concentration above 0.0025% has a significant inhibition on the growth of B. bifidum and has no influence on the other four probiotics at incubation12h or 24h. The optimum selective concentration of kanamycin sulfate in MRS media was 0.005% for selective enumeration of B.bifidum. Addition of gentamicin at the concentration above 240U/ml has a significant inhibition on the growth of L. bulgaricus at incubation 24h. Addition of neomycin sulfate in MRS can use to realize selective enumeration of L. bulgaricus in yogurt

Abstract: The study on the combined probiotics to replace antibiotics becomes more and more important because the by-effects and appearance of “superbug” from abuse of antibiotics in human life and animal production. In order to get the effective combined probiotics for replacing antibiotics and inhibiting pathogenic Escherichia coli (E. coli) prolification, 3 strains of Bacillus subtilis (B. subtilis, named as B1, B2 and B3) were used to determine the pre-combination with experimental design (3 factors×4 levels), and then the pre-combination of B. subtilis was combined with Lactobacillus casei (L. casei) and Hansenula anomala (H. anomala) to achieve the most effective combined probiotics with the same design. The results indicated that the pre-combination ratio of B1, B2 and B3 was 0:1:1, and the most effective combination ratios of the pre-combination, L. casei and H. anomala were 0:1:1, 1:3:0, 2:2:3, 2:3:2, respectively (P<0.05). In addition, the incubating groups containing L. casei had better effect on inhibiting E. coli prolification than those without Lactobacillus, indicating that Lactobacillus had the best effect on inhibiting E. coli prolification, compared with other bacteria.

Abstract: Effect of bile and nalidixic acid on growth of Lactobacillus acidophilus, Bifidobacterium bifidum, Lactobacillus bulgaricus, Lactobacillus casei and Lactobacillus rhamnosus was studied by measuring the optical density at 600nm (OD600) and pH using De Man, Rogosa and Sharpe (MRS ) broth as the control. The addition of bile (%, w/v) was 0.03%, 0.06%, 0.09%, 0.12% and 0.15% and the addition of nalidixic acid (%, w/v) was 0.003%, 0.009%, 0.015%, 0.045% and 0.075%. Results were as follows: addition of bile at the concentration above 0.06% has a significant inhibition on the growth of L. bulgaricus and B. bifidum at incubation 24h. The optimum selective concentration of bile in MRS media was 0.06% for L. bulgaricus and B. bifidum. Addition of nalidixic acid has no effect on the growth of the five selected probiotics at incubation 24h. Addition of nalidixic acid in MRS can not use to realize selective enumeration in yogurt product containing probiotics.

Abstract: Effect of the extract of Fructus tribuli (EFT) on growth of Lactobacillus acidophilus LA04, LA05, LA06 and LA09 was studied by measuring optical density at 600nm (OD600) and pH using MRS culture media as the control. The addition of EFT (v/v) was 1%, 2%, 3%, 4% and 5%. Results were as follows: addition of EFT could promote the growth of Lactobacillus acidophilus LA09, The optimum concentration of EFT in MRS media was 1% at incubation 24h, but addition of EFT on growth of Lactobacillus acidophilus LA04, LA05 and LA06 has no significant effect.

Abstract: Effect of steroidal saponins fromFructus tribuli on growth of Bifidobacterium bifidum BB01, BB03 and Lactobacillus acidophilus LA09 was studied by measuring optical density at 600nm (OD600) and pH using MRS media as the control. The concentration of steroidal saponins (w/v) added in was 0.05%, 0.10%, 0.15%, 0.20% and 0.25%. Results were as follows: addition of steroidal saponins could improve the buffer ability of MRS and promote the growth of Bifidobacterium bifidum BB01 and BB03. The optimum concentration of nutrient supplement in MRS media was 0.20% at incubation 24h for Bifidobacterium bifidum BB01 and 0.25% at incubation 24h for Bifidobacterium bifidum BB03. Addition of steroidal saponins on Lactobacillus acidophilus LA09 didn’t play an obvious role in promoting growth when it was in high level.