Papers by Keyword: Protein Adhesion

Abstract: Protein adhesion on biomaterial surfaces plays a major role in determining their biocompatibility and cell responses. The goal of this study was to produce chitosan-based coatings of implant material polyurethane (PUR) for reduced human serum albumin (HSA) adhesion. Succinic anhydride was employed for modifying chitosan and synthesis of N-succinyl-chitosan (NSCS) which was used as a matrix coating of PUR. NSCS showed self-assembly behaviour as nanofiber structures onto PUR surface. Atomic force microscopy (AFM) has emerged as useful tool for the molecular force measurements and therefore it has been chosen to investigate the adhesion properties of Human serum albumin (HSA) on the new matrix coatings and other three implant materials PUR, Silicon and Titanium. HSA molecules were covalently bound to the AFM tip by the use of cyanuric chloride as bivalent linker. Analyzing the force curves demonstrated the antiadhesive properties of the NSCS films in comparison with the uncoated PUR, Silicon and Titanium.

Abstract: A new, direct method has been developed to measure the adhesion forces of bovine serum albumin (BSA) on surfaces by using Atomic Force Microscopy (AFM) in liquid environment. We were able to measure interactions between proteins and substrate surface in PBS solution directly without any modification to the substrate or the AFM tip. Two different surfaces have been used in the experiments: mica (hydrophilic surface) and polystyrene (hydrophobic surface). The results show that a polystyrene surface is more adhesive to BSA than a mica surface. This is consistent with previous research, which assessed that hydrophobic surfaces enhance protein adhesion but hydrophilic surfaces do not, demonstrating the effectiveness of the technique.