Papers by Keyword: Sarafloxacin

Abstract: During cold preservation, the pH and moisture of beef decreased slowly. Changes in ash and protein content had no obvious rules, but the data analysis results showed that the effect of drugs on these indexes was significant. Ca, Fe content decreased, Zn content increased, TBARS values were significantly higher than those in the blank , and violent fluctuation. SAR and DIF had obvious bacteriostasis effect. Cold preservation had no palpable effect on fluoroquinolones residues.

Abstract: Through cell fusion technology, five hybridoma lines of sarafloxacin (SAR), named S1-B2, S2-C6, S2-E7, S3-C5, and S3-E5, were identified and their corresponding mAbs were of the IgG1 isotype with a k light chain. The Kaffs of all mAbs were between 2.8 and 4.6×109 L/mol. The titers and IC50 values of purified ascite fluids were in the range of 0.512–2.56×106 and 0.32–0.48 ng/mL, respectively. The performances of S1-B2 and S2-C6 were more consistent in the stability experiments. Based on the S1-B2 hybridoma, an icELISA method was developed. The dynamic range was from 0.004 to 18 ng/mL, with a detection limit for the assay and IC50 values of 0.002 and 0.32 ng/mL, respectively. Therefore, the establishment of these hybridomas may provide an alternative method for the detection of SAR residues in food-original animals.