Papers by Keyword: Slip Trace

Abstract: Bone microstructure is dominantly composed of anisotropic extracellular matrix (ECM) in which collagen fibers and epitaxially-oriented biological apatite (BAp) crystals are preferentially aligned depending on the bone anatomical position, resulting in exerting appropriate mechanical function. The regenerative bone in bony defects is however produced without the preferential alignment of collagen fibers and the c-axis of BAp crystals, and subsequently reproduced to recover toward intact alignment. Thus, it is necessary to produce the anisotropic bone-mimetic tissue for the quick recovery of original bone tissue and the related mechanical ability in the early stage of bone regeneration. Our group is focusing on the methodology for regulating the arrangement of bone cells, the following secretion of collagen and the self-assembled mineralization by oriented BAp crystallites. Cyclic stretching in vitro to bone cells, principal-stress loading in vivo on scaffolds, step formation by slip traces on Ti single crystal, surface modification by laser induced periodic surface structure (LIPSS), anisotropic collagen substrate with the different degree of orientation, etc. can dominate bone cell arrangement and lead to the construction of the oriented ECM similar to the bone tissue architecture. This suggests that stress/strain loading, surface topography and chemical anisotropy are useful to produce bone-like microstructure in order to promote the regeneration of anisotropic bone tissue and to understand the controlling parameters for anisotropic osteogenesis induction.

Abstract: Cells are known to sense the topographic features of the substrate and align along the direction of the surface pattern, and this is believed to be an important aspect in the formation and regeneration of anisotropic biological tissues. In this study, a unique and anisotropic stepped pattern was produced on single crystals of α-Ti with the h.c.p. lattice by plastic deformation in compression to demonstrate the effect of the pattern on cell behavior. Because the Schmid factor for the operative slip system of prismatic (100)[110] was set to be 0.5, the slip traces with an acute angle of 45° appeared on the surface. A smooth substrate without plastic deformation was used as a control. MC3T3-E1 osteoblastic cells were cultured on the substrate for 24 h, followed by observation of the morphology and alignment of the cells by Giemsa staining. On stepped substrates, cells aligned along the slip traces, and the filopodia of the aligned cells were found to extend parallel to the slip traces. The slip traces induced by plastic deformation of a single crystal was successfully proven to be a potent substrate to control the alignment of cells.

Abstract: The positive temperature dependence of flow stress in Ni3Al is examined through fine slip trace analysis performed by atomic force microscopy. Slip traces, which result from dislocation movements, constitute outstanding markers for investigating the elementary dislocation mechanisms that control plasticity. The experiments were performed on two Ni3Al-base alloys, with Ta or Hf as additional elements. The results give evidence that the anomaly domain is accompanied by a drastic exhaustion of mobile dislocations and very short cross-slip distances on the cube cross-slip plane.