Papers by Keyword: bFGF

Abstract: Objective: To investigate effects of bFGF and collagen on tissue-engineered cartilage in pellet culture. Methods: Rabbit rib growth-plate chondrocytes were incubated in pellet culture. bFGF and Collagen was applied alone and in combination based on the results of previous search . The histological structure of neocartilage was observed by species stained with H＆E and Immunohistochemical analysis. The DNA content of neocartilage was measured by hoechst33258 dye. The production of matrix was estimated from the determination of hydroxyproline content and Alcian Blue method. Results: Chondrocytes formed cartilage-like tissue with distinct differentiated zones and regions. The DNA content of neocartilage was enhanced by bFGF and collagen. The production of collagen and PG was promoted by collagen. bFGF and collagen could suppress the expression of collagenⅠwhile they could decelerate the decrease of collagenⅡ. The combination of bFGF and collagen had synergistic effects. Conclusion: Pellet culture could maintain chondrocytes phenotype and enhance matrix production; bFGF could stimulate the proliferation of chondrocytes, collagen could stimulate the proliferation and the matrix production of chondrocytes, it also had a positive effect on the maintenance of cell phenotype, the combination of bFGF and collagen had synergistic effects on the maintenance of chondrocytes phenotype and the regeneration of cartilage.

Abstract: The porous neutralized chitosan scaffold (NCS) was prepared by freeze-dry method. Its poor cell binding capacity was improved approximately five folds by mixing or coating of atelomeric type I collagen. In order to recreate wound-healing microenvironment within the NCS for the better wound healing effect, various concentrations of bFGF and fibronectin (FN) were supplied in the secondary freeze-dry process of the scaffold. NCS+ bFGF and NCS+FN improved the cell binding capacity by four folds and three folds respectively. Therefore supplementation of collagen, b-FGF and/or fibronectin in the NCS can improve the biocompatibility of the chitosanbased scaffold which itself revealed poor cell binding capacity.

Abstract: A novel composite of biodegradable Poly-L-lactic acid (PLLA) with the deposition of the nanosized amorphous calcium phosphate (NCP) particles was developed as tissue engineering scaffold. To improve the minor intrinsic healing capacity of cartilage tissue, the porous composite with desired degradation rate was incorporated with basic fibroblast growth factor (bFGF) and evaluated in the in vivo environment. Full-thickness defects were created in the weight-bearing surface of the femoral condyles in a rabbit model. The defect was filled with and without NCP/PLLA scaffold as a carrier of bFGF. Gross morphology for the test implant showed that the defect was filled with regenerated tissue. It resembled cartilaginous tissue and restored the contour of the condyle at 8 weeks after operation. For the untreated control, no cartilage-like tissue was observed at all defects. Histological analysis revealed neochondrogenesis and clusters of cartilaginous extracellular matrix observed with safranin-O staining at 4 weeks for the NCP/PLLA with bFGF treated defects. At 8 weeks after operation, well-formed and mature cartilage was resurfaced the defects. While only fibrous tissue replacement was observed for the control either at 4 or 8 weeks. Special staining for cartilage indicated the presence of highly sulfated glycosaminoglycans and collagen, which were the major extracellular matrices of cartilage. This investigation showed the potential of NCP/PLLA loaded with bFGF in the study of in situ-transplantable carrier to improve healing of cartilage tissue lesion.

Abstract: The objective of this study is to investigate the proliferation and differentiation of stromal cells derived from human fat tissues cultured on substrates with different surface properties. In addition, the similar investigation was performed for the cells proliferated in different concentrations of basic fibroblast growth factor (bFGF). The culture substrates include several polymer films with different water wettabilities, glass or a cell culture plate, and that coated with collagen type I or IV, gelatin, and bFGF. The proliferation profiles of cells were influenced by the type of culture substrates and the growth factor concentration. A larger number of cells proliferated was observed for substrates with the water wettability around 80o, while the cell number was significantly larger for every protein-coated substrate. The rate of cell proliferation became maximum in a bFGF concentration of 1,000 ng/mL. The bFGF concentration used for cell proliferation affected the differentiation profile of cells proliferated. The stromal cells proliferated in 1 ng/mL bFGF were osteogenically differentiated to the strongest and fastest extent among those in other growth factor doses. The alkaline phosphatase (ALP) activity of cells increased with the increased cell number although the activity per cells was indentical, irrespective of the substrates type. The strongest adipogenic differentiation was observed for cells proliferated in 1,000 ng/mL bFGF and the differentiation induction was maintained for a long time period. No clear dependence of the cell number on adipogenesis was observed. For chondrogenic differentiation, the bFGF concentration had no influence on the glycosaminoglycans (GAG) amount. These findings indicate that the proliferation and differentiation of human fat tissue-derived stromal cells are influenced by the culture substrate and the concentration of bFGF used for proliferation.