Paper Titles
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p.1091
OPG and Rankl Expression in Osteoblasts Grown on Different HA Ceramics
p.1095
A Novel Collagen Sponge for Cartilage Tissue Engineering
p.1101
Effect of a Single Period of Mechanical Strain on Gene Expression Patterns of Ets1 and Cbfa1 in Murine Calvarial Sutural Osteoblast-Like Cells
p.1105
Ectopic Osteogenesis of Bone Marrow Stromal Cells Loading in Hydroxyapatite/ β-Tricalcium Phosphate
p.1109
The Optimized Design of a Double-Layer Flow Chamber
p.1113
Calcium Phosphate Hybridized with Human Semitendinosus and Gracilis Tendon Grafts
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Repairing Periodontal Bone Defect with In Vivo Tissue Engineering Bone
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Proliferation Effect of Mandibular Condylar Chondrocytes Given The Static Tension-Stress and/or TGF-β1 In Vitro
p.1125

Ectopic Osteogenesis of Bone Marrow Stromal Cells Loading in Hydroxyapatite/ β-Tricalcium Phosphate

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Abstract:

This study was to determine the ectopic osteogenic ability of BMSCs in combination with a scaffolding material comprising hydroxyapatite and β-tricalcium phosphate matrix (HA/β-TCP). BMSCs were obtained from the SD rats and induced to osteogenesis. Then these induced cells were seeded into HA/β-TCP and the constructs were auto-implanted subcutaneously for up to 12 weeks. Histological analysis, immunostaing, RT-PCR and transmission electron microscopy of the retrieved specimens at various intervals showed obvious trends of ectopic bone formation with obvious alteration of cellular phenotype.

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Periodical:

Key Engineering Materials (Volumes 330-332)

Pages:

1109-1112

DOI:

https://doi.org/10.4028/www.scientific.net/KEM.330-332.1109

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Online since:

February 2007

Authors:

Yun Feng Lin, Ling Wu, Lei Liu, Ju Qiao, Wei Jing, Wei Dong Tian

Keywords:

Bone Marrow Stromal Cell, Bone Tissue Engineering, Hydroxyapatite/ß-Tricalcium Phosphate, Osteogenic Differentiation

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© 2007 Trans Tech Publications Ltd. All Rights Reserved

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References

[1] Kajiwara R, Ishida O, and Kawasaki K: J Orthop Res, Vol. 23 (2005), p.909.

Google Scholar

[2] Mauney JR, Volloch V, and Kaplan DL: Tissue Eng, Vol. 11(2005), p.787.

Google Scholar

[3] Bosnakovski D, Mizuno M, and Kim G: Cell Tissue Res, Vol. 319(2005), p.243.

Google Scholar

[4] Seitz H, Rieder W, and Irsen S: J Biomed Mater Res B Appl Biomater. Vol. 74B (2005), p.782.

Google Scholar

[5] World Medical Association; American Physiological Society. Guiding principles for research involving animals and human beings. Am J Physiol Regul Integr Comp Physiol, Vol. 283 (2002), p.281.

Google Scholar

[6] Lin Y, Chen X, and Tian W: Mol Cell Biochem. Vol. 285(2006), p.69.

Google Scholar

[7] Lin Y, Yan Z, and Liu L: Cell Prolif. Vol. 39(2006), p.79.

Google Scholar

[8] Zohar R, Cheifetz S, and McCulloch CA: Eur J Oral Sci, Vol. 106(1998), p.401.

Google Scholar

[9] Morinobu M, Ishijima M, and Rittling SR: J Bone Miner Res, Vol. 18(2003), p.1706.

Google Scholar