Engineering Biomaterials Surfaces Using Micropatterning

Louis Gagne; Gaetan Laroche

doi:10.4028/www.scientific.net/AMR.15-17.77

Paper Titles

Influence of SO₂ Concentration on Initial Corrosion of Aluminum in Marine Atmosphere
p.53

Influence of Cooling Rate on the Size of the Precipitates and Thermal Characteristic of Al-Si Cast Alloys
p.59

Bone Tissue Engineering: Natural Origination or Synthetic Polymeric Scaffolds?
p.65

Preparation and Characterisation of New Titanium Based Alloys for Orthopaedic and Dental Applications
p.71

Engineering Biomaterials Surfaces Using Micropatterning
p.77

Atomic Force and Confocal Microscopic Studies of Collagen-Cell-Based Scaffolds for Vascular Tissue Engineering
p.83

Surface Modification of TiZr Alloy for Biomedical Application
p.89

Real-Time PCR Quantification of Protein Expression in Skin Equivalent Monoculture and Coculture Model
p.95

Tissue Engineering of Vascular Graft
p.101

HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 15-17Engineering Biomaterials Surfaces Using...

Engineering Biomaterials Surfaces Using Micropatterning

Abstract:

A new technique for micropatterning surfaces for cell growth support is described and characterized. This technique allows covering of large three-dimensional surfaces at low cost with controllable micropatterns. This method takes advantage of the random properties of aerosols and the principles of liquid atomization. Parameters of interest were the pressure of atomization air, the flow rate and volume of the atomised liquid, and the distance between the spray nozzle and the surface of the sample. The experimental setup permitted to obtain mean diameters of spots between 10 and 20 microns with a maximum surface coverage of 20%. In an initial step, polytetrafluoroethylene (PTFE) films were treated with ammonia plasma to insert amino groups on the surface. The ammonia plasma treated films were immersed in a solution containing sulfosuccinimidyl 4-(N-maleidomethyl)cyclohexane-1-carboxy-late (SSMCC) to permit the introduction of maleimido groups on the PTFE surface to subsequently conjugate peptides through a sulfhydryl containing N-terminal cystein residue. Plasma/S-SMCC pretreated surfaces were then sprayed with peptide sequences CGRGDS and CWQPPRARI. Our data showed that spots of CGRGDS peptides over a background of CWQPPRARI peptides were the most effective combination to enhance endothelialization.