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Reconstruction of Kidney-Like Tissue Using ECM Gel and Rat Embryonic Kidney Fragments
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Reconstruction of Kidney-Like Tissue Using ECM Gel and Rat Embryonic Kidney Fragments

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Abstract:

Organ engineering remains a challenge to researchers. We tried to reconstruct kidney-like tissue using tissue engineering technique. Kidney fragments were isolated from rat E16 embryonic kidneys and seeded on either ECM gel or on polyglycolic acid (PGA) fibers, then implanted in vivo into the subcutaneous tissue of nude mice for 2 and 3 weeks, respectively. As a negative control, ECM alone was implanted. Results showed that kidney like tissue could be formed in ECMfragment constructs after 2 or 3 weeks of implantation, including the formation of glomeruli, tubules and collecting ducts. In addition, these structures seemed more mature in 3 weeks specimens than that of 2 weeks. Further development of this model may lay a solid foundation for kidney tissue engineering.

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Periodical:

Key Engineering Materials (Volumes 342-343)

Pages:

1-4

DOI:

https://doi.org/10.4028/www.scientific.net/KEM.342-343.1

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Online since:

July 2007

Authors:

Ying Jian Zhu, Wei Liu, Wen Jie Zhang, Guang Dong Zhou, Guo Hua Liu, Yi Lin Cao

Keywords:

ECM Gel, Kidney-Like Tissue, PGA

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© 2007 Trans Tech Publications Ltd. All Rights Reserved

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