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Glucosylated Polypropylenimine Dendrimer as a Novel Gene Carrier

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Abstract:

Polypropylenimine (PPI) dendrimers have been used by many researchers as gene delivery carriers due to their high functionality. Glucose as a kind of carbohydrate is biocompatible and hydrophilic. In this study, we synthesized glucosylated PPI (G-PPI) dendrimers to reduce cytotoxicity. Glucose substitution of G-PPI dendrimers was determined by the sulfuric acid micromethod. The G-PPI dendrimer was complexed with plasmid DNA in various N/P ratios, and the complex was characterized. G-PPI dendrimers showed good DNA binding ability and high protection of DNA from nuclease attack. The G-PPI dendrimer had low cytotoxicity compared to PPI dendrimer by cytotoxicity assay. Also, transfection efficiency was influenced by glucosylation degree and the transfection efficiency for the G-PPI-5 was slightly higher than that of PPI dendrimer in HeLa cell line.

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Periodical:

Key Engineering Materials (Volumes 342-343)

Pages:

457-460

DOI:

https://doi.org/10.4028/www.scientific.net/KEM.342-343.457

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Online since:

July 2007

Authors:

You Kyoung Kim, In Kyu Park, Hu Lin Jiang, Rohidas B. Arote, Hwan Jeong Jeong, Eun Mi Kim, Myung Haing Cho, Hee Seung Bom, Chong Su Cho

Keywords:

Cytotoxicity, Gene Delivery, Glucosylation, Polypropylenimine (PPI) Dendrimer

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© 2007 Trans Tech Publications Ltd. All Rights Reserved

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