Paper Titles
Coating of Anionic Phospholipid Polymer onto Silica-Based Microchannel to Minimize Nonspecific Protein Adsorption
p.797
Improving Blood Compatibility of Cardiovascular Devices by Surface Modification
p.801
Blood Compatibility of Novel Zwitterionic PEG-Grafted Nitinol Alloy for Peripheral Arterial Stents
p.805
Ozone-Induced Immobilization of Chitosan and Heparin on Polyethylene Terephthalate Films to Improve Antithrombogenic Properties
p.809
Effect of Solvent on the Characteristics of Electrospun Regenerated Silk Fibroin Nanofibers
p.813
Preparation and Characterization of β-Tricalcium Phosphate Nanofibers via Electrospinning
p.817
Occlusion Effect of Dentinal Tubules of the Dentifrice Containing Nano-Sized Carbonate Apatite
p.821
Study of Protein Adsorption on Biomaterial Surfaces by SPR
p.825
Improvement and FEA of New Type of Osteointegrated Implant for Higher Amputated Leg Attachment
p.829

Effect of Solvent on the Characteristics of Electrospun Regenerated Silk Fibroin Nanofibers

Article Preview

Abstract:

Nonwoven nanofiber matrices were prepared by electrospinning a solution of silk fibroin (SF) dissolved either in formic acid or in 1,1,1,3,3,3-hexafluoro-2-isopropyl alcohol (HFIP). The mean diameter of the electrospun nanofibers prepared from SF dissolved in formic acid was 80 nm with a unimodal size distribution, which was smaller than those prepared from HFIP (380 nm). SF nanofibers were then treated with an aqueous methanol solution, and structural changes due to solvent-induced crystallization of SF were investigated using IR and 13C solid-state CP/MAS NMR spectroscopy. SF nanofibers prepared from formic acid were found to have a higher proportion of β-sheet conformations than those prepared from HFIP. Methanol treatment provided a fast and effective means to alter the secondary structure of both types of SF nanofibers from a random coil form to a β-sheet form. As demonstrated in the present study, this approach to controlling the dimensions and secondary structure of proteins using various solvents may be useful for the design and tailoring of materials for biomedical applications, especially for tissue engineering applications.

You might also be interested in these eBooks
Advanced Biomaterials VII View Preview

Info:

Periodical:

Key Engineering Materials (Volumes 342-343)

Pages:

813-816

DOI:

https://doi.org/10.4028/www.scientific.net/KEM.342-343.813

Citation:

Cite this paper

Online since:

July 2007

Authors:

Lim Jeong, Kuen Yong Lee, Won Ho Park

Keywords:

Electro-Spinning, Nanofiber, Secondary Structure, Silk Fibroin, β Sheet

Export:

RIS, BibTeX

Price:

Permissions CCC:

Request Permissions

Permissions PLS:

Request Permissions

Сopyright:

© 2007 Trans Tech Publications Ltd. All Rights Reserved

Share:

Citation:

References

[1] Li, S. Lu, Z. Wu, K. Tan, N. Minoura and S. Kuga: Int. J. Biol. Macromol. Vol. 30 (2002), p.89.

Google Scholar

[2] J. Yao, H. Masuda, C. Zhao and T. Asakura: Macromolecules Vol. 35 (2002), p.6.

Google Scholar

[3] K. Cai, K. Yao, S. Lin, Z. Yang, X. Li, H. Xie, T. Qing and L. Gao: Biomaterials 23 (2002), p.1153.

Google Scholar

[4] B.M. Min, L. Jeong, Y.S. Nam, J.M. Kim, J.Y. Kim and W.H. Park: Int. J. Biol. Macromol. Vol. 34 (2004), p.281.

Google Scholar

[5] V. Karageorgiou, L. Meinel S. Hofmann, A. Malhotra, V. Volloch and D. Kaplan: J. Biomed. Mater. Res. Vol. 71A (2004), p.528.

DOI: 10.1002/jbm.a.30186

Google Scholar

[6] B.M. Min, G. Lee, S.H. Kim, Y.S. Nam, T.S. Lee and W.H. Park: Biomaterials Vol. 25 (2004), p.1289.

Google Scholar

[7] M. Ishida, T. Asakura, M. Yokoi and H. Saito: Macromolecules Vol. 23 (1990), p.88.

Google Scholar

[8] B.M. Min, L. Jeong, K.Y. Lee and W.H. Park: Macromol. Biosci. Vol. 6 (2006), p.285.

Google Scholar

[9] D. Wilson, R. Valluzzi and D. Kaplan: Biophys J. Vol. 78 (2000), p.2690.

Google Scholar