Paper Titles
Interactions of Acidithiobacillus ferrooxidans with Heavy Metals, Various Forms of Arsenic and Pyrite
p.423
Regulation of the Arsenic Oxidation Encoding Genes of a Moderately Acidophilic, Facultative Chemolithoautotrophic Thiomonas sp.
p.427
Ferrous Iron Oxidation by Salt-Tolerant “Thiobacillus prosperus”
p.431
Differential Genetic Expression in Heap Bioleaching of Low-Grade Copper Sulphide Ore at Escondida Mine, Chile
p.435
Insights into the Metabolism and Ecophysiology of Three Acidithiobacilli by Comparative Genome Analysis
p.439
Involvement of Iron Oxidation Enzyme System in Sulfur Oxidation of Acidithiobacillus ferrooxidans ATCC 23270
p.443
The Effect of Total Iron Concentration and Iron Speciation on the Rate of Ferrous Iron Oxidation Kinetics of Leptospirillum ferriphilum in Continuous Tank Systems
p.447
Identification, Some Characterizations and Bioleaching Application of an Iron-Oxidizing Bacterium, Acidithiobacillus ferrooxidans KLipis-3-1 Isolated from Malaysia
p.453
Quantification of Biomining Microorganisms Using Quantitative Real-Time Polymerase Chain Reaction
p.457

Insights into the Metabolism and Ecophysiology of Three Acidithiobacilli by Comparative Genome Analysis

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Abstract:

Draft genome sequences of Acidithiobacillus thiooxidans ATCC 19377 and A. caldus ATCC 51756 have been annotated. Bioinformatic analysis of these two new genomes, together with that of A. ferrooxidans ATCC 23270, allows the prediction of metabolic and regulatory models for each species and has provided a unique opportunity to undertake comparative genomic studies of this group of bioleaching bacteria. In this paper, we report preliminary information on metabolic and electron transfer pathways for ten characteristics of the three acidithiobacilli: CO2 fixation, the TCA cycle, sulfur oxidation, sulfur reduction, iron oxidation, iron assimilation, hydrogen oxidation, flagella formation, Che signaling (chemotaxis) and nitrogen fixation. Predicted transcriptional and metabolic interplay between pathways pinpoints potential coordinated responses to environmental signals such as energy source, oxygen and nutrient limitations. The predicted pathway for nitrogen fixation in A. ferrooxidans will be described as an example of such an integrated response. Several responses appear to be especially characteristic of autotrophic microorganisms and may have direct implications for metabolic processes of critical relevance to the understanding of how these microorganisms survive and proliferate in extreme environments, including industrial bioleaching operations.

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Periodical:

Advanced Materials Research (Volumes 20-21)

Pages:

439-442

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.20-21.439

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Online since:

July 2007

Authors:

Jorge H. Valdés, Inti Pedroso, Raquel Quatrini, Kevin B. Hallberg, Pablo D.T. Valenzuela, David S. Holmes

Keywords:

Acidithiobacillus, Bioinformatics, Comparative Genomics, Metabolic Integration

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© 2007 Trans Tech Publications Ltd. All Rights Reserved

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