Paper Titles
Is Hydroxyapatite Ceramic Included in the Bone Remodelling Proccess? An In Vitro Study of Resorption and Formation Processes
p.1123
In Vitro and In Vivo Biological Behavior of CDHA/OCP/β-TCP Scaffold
p.1127
Cell Growth on TiAlNb Alloy as a Function of Bioactivation Method
p.1131
Cytotoxicity Analysis of Apatites Modified with Divalent Metals
p.1135
Effect of Sintering Process of HA/TCP Bioceramics on Microstructure, Dissolution, Cell Proliferation and Bone Ingrowth
p.1139
Characterization of Bone Cell Behaviors on Collagen Grafted Hydroxyapatite Surfaces
p.1143
Isolation Yield and Osteogenic Potential of Human Bone Marrow Stromal Cells are Maintained Irrespective of Age or Gender
p.1149
Expression Patterns of Cell Surface Molecules on Human Bone Marrow Stromal Cells from Multi-Donors
p.1153
Mechanical Properties of Living Cells: Interaction of the Adherent Endothelial Cell and the Fluid Shear Flow
p.1157

Effect of Sintering Process of HA/TCP Bioceramics on Microstructure, Dissolution, Cell Proliferation and Bone Ingrowth

Article Preview

Abstract:

The purpose of this study was to determine the effect of sintering conditions on microporosity of and cell proliferation and bone ingrowth on biphasic calcium phosphate (BCP) bioceramics. Discs were prepared from a calcium-deficient apatite preparation that upon sintering at 1050oC and above, results in a BCP with 60% hydroxyapatite (HA)/ 40% beta-tricalcium phosphate (β-TCP) ratio. The discs were divided into groups which were sintered under different conditions of heating rate (programmed vs. non-programmed) and temperature (1050°C vs. 1200°C). The discs were characterized in terms of composition (HA/β-TCP ratio), surface morphology, surface area, surface microporosity, per cent microporosity, and dissolution properties. The in vitro effect of sintering conditions on cell proliferation was determined using an established mouse fibroblast cell line (L929). Results demonstrated the following: (a) the HA/β-TCP ratio remained 60/40 regardless of sintering conditions; (b) the % microporosity, surface microporosity, surface area of the BCP and cell proliferation on the BCP significantly decreased with increasing sintering temperature, and (c) the extent of dissolution decreased with decreasing per cent microporosity. The in vivo study indicated no tissue adverse reaction and direct bone contact with the implant surface, confirming the biocompatibility of the BCP bioceramics. Resorption of the BCP and bone ingrowth was directly related to the sintering temperature: the higher the temperature, the lower the resorption and the bone ingrowth. Results of this study indicate that per cent microporosity of the BCP bioceramics affects its dissolution properties and cell response. The study demonstrates that optimum per cent microporosity elicits optimum cell response and should be considered to provide osteogenic/osteoinductive property to bioceramics.

You might also be interested in these eBooks
Bioceramics 20 View Preview

Info:

Periodical:

Key Engineering Materials (Volumes 361-363)

Pages:

1139-1142

DOI:

https://doi.org/10.4028/www.scientific.net/KEM.361-363.1139

Citation:

Cite this paper

Online since:

November 2007

Authors:

Guy Daculsi, Racquel Z. LeGeros, Gael Grimandi, A. Soueidan, Eric Aguado, Eric Goyenvalle, John P. LeGeros

Keywords:

Biphasic Calcium Phosphate (BCP), Bone Ingrowth, Cell Proliferation, Microstructure, Sintering

Export:

RIS, BibTeX

Price:

Permissions CCC:

Request Permissions

Permissions PLS:

Request Permissions

Сopyright:

© 2008 Trans Tech Publications Ltd. All Rights Reserved

Share:

Citation:

References

[1] LeGeros RZ, Lin S, Rohanizadeh R, Mijares D, LeGeros JP (2003). J Mater Sci Mat Med 14: 201-209.

DOI: 10.1023/a:1022872421333

Google Scholar

[2] LeGeros RZ, Daculsi G (1990). In Handbook of Bioactive Ceramics, Vol II: Calcium Phosphate Ceramics. Yamamuro N, Hench L, Wilson-Hench J (Eds): CRC Press: Boca Raton; FL, pp.17-28.

DOI: 10.1002/jbm.820250709

Google Scholar

[3] Daculsi G, Laboux O, Malard O ; Weiss P (2003). J Mat Sci: Mat 14 : 195-200.

Google Scholar

[4] Kuboki Y, Takita H, Kobayashi D. J Biomed Mater Res 39: 190-199.

Google Scholar

[5] Ripamonti U (1995). Biomaterials 17: 31-35.

Google Scholar

[6] Reddi AH (2000). Tissue Eng 6351-359.

Google Scholar

[7] Hulbert SF, Morrison SJ; Klawitter JJ (1973). J Biomed Mater Res 6: 347-374.

Google Scholar

[8] Li S, de Wijn JR, Li J, Layrolle P,; de Groot K (2003). Tissue Eng 9 : 535-548.

Google Scholar

[9] Daculsi G, Layrolle P. (2004) Key Engineering Materials. 2004; 254-256: 1005-8.

Google Scholar

[10] Le Nihouannen D, Daculsi G, Saffarzadeh A, Gauthier O, Delplace S, Pilet P, Layrolle P (2005) Bone 36: 1086-93.

DOI: 10.1016/j.bone.2005.02.017

Google Scholar

[11] LeGeros RZ (1993). Clin Mat 14: 65-88.

Google Scholar

[12] LeGeros RZ (2002). Clin Orthopaed Rel Res 395: 81-98.

Google Scholar

[13] Heughebaert M, LeGeros RZ, Gineste M, Bonel G (1988). J biomed Mater Res 23: 257-268.

DOI: 10.1002/jbm.820221406

Google Scholar

[14] Daculsi G, LeGeros RZ, Heugheaert M, Barbieux I (1990). Calcif Tissue Int 46: 20-27.

Google Scholar