Paper Titles
A Simple but Effective Demineralizing Method for Osteogenic Allograft Preparation
p.249
Bone Induction by Controlled Release of BMP-2 from a Biodegradable Hydrogel in Various Animal Species - From Mouse to Non-Human Primate -
p.253
Fibrin-Reinforced Collagen Matrix Enhances Tissue Regeneration and Angiogenesis
p.257
The Study of Collagen-HA Composite with Incorporated BMP as Bone Tissue Engineering Scaffold
p.261
Gene Expression of C2C12 Cells on Calcium Phosphate Ceramic In Vitro
p.265
Evaluation of Gamma Irradiated Human Bone in Nude Rats
p.269
Influence of Dynamic Flow Speed on Bonelike Apatite Formation in Porous Calcium Phosphate Ceramic in RSBF
p.273
Nucleation and Growth of Bone-Like Apatite on Surfaces of Metals, Ceramics and Polymers in Simulated Body Fluids
p.277
Experimental Study on the Osteoinduction of Calcium Phosphate Biomaterials In Vivo and the Capability of Supporting Osteoblast Proliferation In Vitro
p.281

Gene Expression of C2C12 Cells on Calcium Phosphate Ceramic In Vitro

Article Preview

Abstract:

The osteoinduction of Calcium Phosphate (CaP) had been proved and generally been investigated by in vivo implantation. However, the mechanism of the osteoinductivity was not clear and it was difficult to judge the osteoinductivity in vitro. In this study, Mouse C2C12 cell line, a kind of myoblast precursor cell, was employed to co-culture with CaP. The induction of cell differentiation by materials was tested by MTT method, fluorescence observation, especially the mRNA expression of Osteocalcin, Type I collagen and Fibronectin by RT-PCR. It was founded that C2C12 cells could be induced to expression osteocalcin when growth on the surface of the HA/TCP ceramics. At the same time, the ceramics with different composition and sintering temperature seemed to induce difference expression level of the related genes. The results proved that phase composition was one of the most important factors in the regulation of bone-related genes. This study provided a potential model to evaluate the osteoinductivity of CaP ceramics in vitro.

You might also be interested in these eBooks
Advanced Biomaterials VI View Preview

Info:

Periodical:

Key Engineering Materials (Volumes 288-289)

Pages:

265-268

DOI:

https://doi.org/10.4028/www.scientific.net/KEM.288-289.265

Citation:

Cite this paper

Online since:

June 2005

Authors:

Yan Fei Tan, Ling Li Zhang, Xin Lai He, Wei Qiang Xiao, Hong Song Fan, Xing Dong Zhang

Keywords:

Calcium Phosphate, Fibronectin, Osteocalcin, RT-PCR, Type I Collagen

Export:

RIS, BibTeX

Price:

Permissions CCC:

Request Permissions

Permissions PLS:

Request Permissions

Сopyright:

© 2005 Trans Tech Publications Ltd. All Rights Reserved

Share:

Citation:

References

[1] Xingdong Zhang, Huipin Yuan, K. de Groot: The 6 th world Biomaterials Congress, May 15-20, 2000, Hawaii, USA.

Google Scholar

[2] Yamasaki H. Herterotopic: Jpn J Oral Biol 1990; 32: 190-192.

Google Scholar

[3] Huipin Yuan, Z. Yang: J. MATER. SCI.: MATER. MED, 9, 723-726, (1998).

Google Scholar

[4] Pei Y et al: Acta Pharmacol Sin 2003 Oct; 24 (10): 975-984.

Google Scholar

[5] Strauss PG, Closs EI, et. al: J Cell Biol 1990; 110: 1369-1378.

Google Scholar

[6] Pockwinse SM, Wilming LG: J Cell Biochem 1992; Vol. 49, pp.310-323.

Google Scholar

[7] Jung-Hwa Rhew1, Young-Ah Shin1: Experimental and Molecular Medicine 1999: Vol. 31, No 2, 71-75.

Google Scholar