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The Transfection Efficacy of Chitosan-DNA Nanoparticles on MGC803, SMMC-7721, MG63 Cells

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Abstract:

Chitosan-DNA nanoparticles were synthesized from the complexation of chitosan solution and the plasmid pCD-GFP encoding green fluorescent protein through a coacervation process. The nanoparticles formed were of diameter inferior to 100 nm. The transfection efficacy of chitosan-DNA nanoparticles in MGC803 cells was higher than in SMMC-7721 cells, whereas the plasmid GFP was not expressed in MG63 cells.

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Periodical:

Materials Science Forum (Volumes 510-511)

Pages:

382-385

DOI:

https://doi.org/10.4028/www.scientific.net/MSF.510-511.382

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Online since:

March 2006

Authors:

Song Chen, Bin Zhang, Lei Ren, Li-Ping Sun, Qi Qing Zhang

Keywords:

Chitosan (CS), GFP, Nanoparticle, Transfection

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© 2006 Trans Tech Publications Ltd. All Rights Reserved

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