Papers by Author: Bo Jiang

Abstract: Collagen has been widely used in biomedical field, such as scaffolds for tissue engineering. However, the rapidly biodegradation and weak mechanical strength of collagen limited its application. In this study, an insoluble collagen extracted from cattle hide was designed as scaffold to act as a three-dimensional substrate for tissue engineering. The received insoluble collagen sponge was analyzed by scanning electron microscopy, infrared spectroscopy, mechanical testing and thermogravimetric-differential thermal analysis. In addition, the degradation was performed in vitro using collagenase. The results showed that the insoluble collagen had the same triple helical domain as acid-soluble collagen, while the compression strength was greatly improved and the degradation rate was reduced. The insoluble collagen sponge with good stability should be promising in tissue engineering scaffold applications.

Abstract: Radiation induced graft polymerization of 2-hydroxyethyl acrylate (HEMA) on titanium surface and couple gelatin have been studied in the paper. The composition and properties of surface modified titanium were investigated by several surface sensitive techniques: X-ray photoelectron spectrum (XPS) and scanning electron microscopy (SEM). Results of the technological measurements show that surface chemistry is affected throughout the surface modification process, finally leading to a complete and homogeneous vinyl compound overlayer on top of the titanium samples. And the modified porous titanium exhibits an interesting mineralization in simulated body fluid (SBF) and an adsorptive property in bovine serum albumin (BSA).

Abstract: As a natural biomaterial, collagen especially pepsin-solubilized collagen (type I) has been used widely in biomedical fields due to its excellent biocompatibility. In this preliminary study, we investigate the effect of some inorganic ions which are frequently utilized in the preparation of collagen on the morphology and crystallinity of fibrils. The scanning electron microscope and x-ray diffraction were applied to analyze the morphology and the crystallization of the reconstituted collagen fibrils, respectively. Although further studies are needed, these initial results indicate that by controlling the self-assembly conditions of collagen molecules, we may achieve the desired properties of fibrillar collagen products.

Abstract: Collagenous molecule was successfully immobilized to hydroxyapatite (HA) surface through a molecular bridge (2-Hydroxyethyl acrylate, HEMA) that was grafted to the surface with covalent bond by gamma irradiation. Hydroxyapatite modified by atelocollagen had been characterized by several surface sensitive techniques, such as FT-IR, SEM, XPS. The investigations showed that the collagen, a bioactive macromolecule, was immobilized on the HA surface through covalent bond.

Abstract: In order to develop a bone-filling material with osteoinductive potential, a composite micorspheres of collagen molecules and biphasic calcium phosphate (BCP) was prepared by utilizing emulsion polymerization and the intrinsic self-assembly of collagen. The prepared microspheres were analyzed by granularity test, scanning electron microscopy (SEM), infrared spectra (IR) and enzymatic digestion experiment. The results showed that the collagen matrix of fibrils was reconstituted in the droplets, and the native triple-helix structure of collagen was still maintained. The study provides an effective way to prepare microspheres of collagen and BCP composite.

Abstract: A kind of medical collagen was prepared by hydrogel formation method. Chemical and physical properties were investigated by FTIR, amino acid analysis, SDS-PAGE, carbohydrate content analysis, heavy metal content analysis. Degradation experiments in vivo and subsequent histological investigations were carried out to evaluate the biological performance. The results suggested that the collagen achieved is promising in tissue engineering scaffold materials for a long-term (more than 12 weeks) implantation application.