Papers by Author: Z. Ahmad

Abstract: Fine nanoapatite relics were deposited on glass substrates by electrohydrodynamic atomisation, using nanohydroxyapatite (nHA), nano-carbonated hydroxyapatite (nCHA) and nanosilicon- substituted hydroxyapatite (nSiHA) suspensions. These electrosprayed nanoapatites were evaluated in-vitro using simulated body fluid (SBF) and human osteoblast (HOB) cells. The SBF study revealed that newly-formed apatite layers were observed on the surface of the relics. Furthermore, enhanced HOB cell growth was observed on each of the nanoapatites at all time points. Hence, this work demonstrated that electrosprayed nanoapatites offer considerable potential as biomaterials.

Abstract: The production of nano-scale hydroxyapatite (HA) suspensions to be used for the reticulated foam method of scaffold production was investigated at temperatures of between 10 and 60OC. An increase in reaction temperature was associated with an increase in the particle size and some decrease in the aspect ratio of the particles. Pre-treatment of the polyurethane foam template using PPDS (potassium peroxodisulfate) solution resulted in a significantly improved coating of HA when compared to the untreated samples or those treated with ethanol. Initial trials coating the polyurethane with HA produced at the different reaction temperatures showed a superior coating with the suspension produced at 10OC compared to that at 60OC. A scaffold was produced using the HA suspension produced at room temperature, but further understanding of the suspension properties and the optimum conditions for coating of the PU foam are required for improved mechanical performance.

Abstract: Nano-sized HA (nHA) was applied to the surface of glass and titanium substrates using electrostatic atomisation spray (EAS) deposition. The phase purity of nHA was confirmed by X-ray diffraction. The nHA suspension consisted of rod–like particles 20-30nm in width and 50-100nm in length. The viscosity and conductivity of nHA suspension were 321 mPa s and 5.6 x 10-4 S/m, respectively. EAS of nHA in cone-jet mode was achieved at flow rate of 10-9 m3s-1 with the applied voltage between the needle and the ring-shaped ground electrode of ~6 kV. Micrometer- to submicrometerscaled nHA islands were successfully deposited on the substrate surface. Image analysis showed that the area percentage of nHA increased with deposition time, it covered 50% of the surface area after 10s of spraying. Partial dissolution of nHA was observed after immersion in deionised water for 1 month, particularly on the submicrometer sized nHA islands. Formation of a bone-like apatite layer was found after incubation in simulated body fluid (SBF K9) for 5 days, indicating the high bioactivity of the nHA deposits. In vitro culture with human osteoblast cells showed that the nHA islands were able to support the growth of HOB cells during 7 days of culture; the HOB cell activity increased with culture time as well as EAS deposition time. Immunofluorescence study showed that HOB cells expressed well-organised actin stress fibres on nHA deposited surfaces after 3 days of culture. The result indicated that nHA deposition provided more favourable surfaces for cell attachment. Therefore, electrostatic atomization spray deposition of nHA offers great potential for the creation of bioactive surfaces on bioinert implant surface to provide improved interfacial bonding with host tissues.