Papers by Keyword: Periodontal Regeneration

Abstract: 75:25 poly (DL-lactide-co-ε-caprolactone) electrospun resorbable membranes were prepared by electrospinning and film casting techniques. Primary cultures of human periodontal ligament (HPDL) fibroblasts cells were seeded on the electrospun membranes, film-cast membranes, and control glass cover slips. The number of cell attached was determined at 1, 3, 24 and 72 h after cell seeding. Cell proliferation on the membranes was determined by MTT assay at 1.5 h and 1, 3, 5 and 7 days after cell seeding. The morphology of cells was also determined by SEM. The results indicated that the cell attached number and cell proliferation on the electrospun membranes were significantly higher than that of the film-cast membranes at every time point. From an SEM study, HPDL cells could healthy attach on both, the electrospun membranes and the film-cast membranes. HPDL cells were firmly attached to the membrane matrix in the electrospun membrane group. In the film-cast group, HPDL were loosely attached to the membrane surfaces. In conclusion, an electrospun membrane had better ability to promote HPDL cell attachment and proliferation than did film-cast membranes.

Abstract: This study evaluated periodontal repair and biomaterial reaction following implantation of a newly fabricated calcium phosphate chitosan block bone and chitosan membrane on the regeneration of 1- wall intrabony defects in the beagle dogs. The surgical control group received a flap operation only, while experimental group was treated with calcium phosphate chitosan block bone and/or chitosan membrane. All dogs were uneventful healing without any complication. The subjects were sacrificed 8 weeks after the experimental surgery, and a comparative histological and histometric examination was done. Chitosan membrane group significantly enhanced bone regeneration compared to control, CaP-chitosan block bone and combination group. Chitosan membrane remnants were observed in chitosan and combination group, while CaP-chitosan bone materials were resorbed completely. These results suggest that Chitosan membrnae significantly enhanced bone and cementum formation in advanced periodontal defects.

Abstract: The aim of this study was to evaluate the regenerative effects of chitosan membranes containing tetracycline (TC) applied to surgically created one-wall intrabony defects in beagle dogs. The defects either received chitosan membrane (CH), chitosan membrane containing 0.5%, 1% TC (CH-TC0.5, CH-TC1.0) or flap operation only. The animals were sacrificed 8 weeks after the experimental surgery, and comparative histological and histometric examinations were done. The amount of junctional epithelium migration and connective tissue adhesion did not show any statistically significant differences among the groups. The amount of new cementum regeneration, and new bone regeneration were significantly greater in CH-TC1.0 group than the control (P<0.05). The results suggest that chitosan membrane combined with 1.0% TC may have beneficial effect on the regeneration of bone and cementum in intrabony periodontal defects.

Abstract: GTR treatment alone has not led to predictable new periodontal tissue reconstruction. Recently, various types of tissue substitutes and alloplastic graft have been used in conjunction with the GTR technique. The aim of this study was to evaluate whether nano-hydroxyapatite/Polylactic acid (nHA/PLA) and bone-derived materials would be useful in the treatment of class II furcation, in order to provide an ideal bone replacement material for periodontal treatment. Mucoperiosteal flap was raised on the buccal aspects of the experimental teeth and class Ⅱ furcation defects were created surgically on premolars of each quadrant in 3 dogs. The defects were randomly repaired with nHA/PLA and collagenic membrane, partially deproteinized bone (PDPB) and collagenic membrane. Blank control group was stayed. Speciments obtained in 6 weeks were evaluated with general observation, histological microscopic analysis. The specimens of the implanted groups exhibited periodontal regeneration including newly formed cementum, periodontal ligament and new bone. The new-formed bone area in sites receiving nHA/PLA and PDPB combined with collagen membrane was greater than that in blank group.The new bone area in PDPB was smaller than that in nHA/PLA. nHA/PLA would be better alternative for periodontal regeneration.

Abstract: The purpose of this study is to evaluate the effect of paste type calcium sulfate on the epithelial migration, alveolar bone regeneration, cementum formation and gingival connective tissue attachment in intrabony defect in dogs. These results suggest that the use of paste type calcium sulfate in 3-wall intrabony defects has significant effect on new cementum formation , but doesn't have any significant effect on the prevention of junctional epithelium migration and new bone formation. As a result, the paste type calcium sulfate that is used in this study is suggested to be the material that can have a significant effect on the periodontal healing, if its biocompatibility is improved.